Application of fluorescence in studying therapeutic enzymes

Zhaoshuai Wang, Caihong Li, Yinan Wei

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations

Abstract

Fluorescence spectroscopy is one of the most important techniques in the study of therapeutic enzymes. The fluorescence phenomenon has been discovered and exploited for centuries, while therapeutic enzymes have been used in treatment of disease for only decades. This chapter provides a brief summary of the current applications of fluorescence methods in studying therapeutic enzymes to provide some insights on the selection of proper method tailored to the goal. First a brief introduction about therapeutic enzymes and history of fluorescence were provided, followed by discussions on how fluorescence was applied in the studies. Four popular fluorescence methods are discussed: fluorescence tracing, fluorescence resonance energy transfer (FRET), fluorescence quenching and fluorescence polarization. Selected application of the fluorescence methods in studying therapeutic enzymes are listed, and discussed in details in the following paragraphs.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
Pages105-114
Number of pages10
DOIs
StatePublished - 2019

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1148
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Bibliographical note

Publisher Copyright:
© Springer Nature Singapore Pte Ltd. 2019.

Keywords

  • FRET
  • Fluorescence polarization
  • Fluorescence quenching
  • Fluorescence tracing
  • Therapeutic enzyme

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)

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