Arabidopsis cold shock domain proteins: Relationships to floral and silique development

Kentaro Nakaminami, Kristine Hill, Sharyn E. Perry, Naoki Sentoku, Jeffrey A. Long, Dale T. Karlson

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Cold shock domain proteins (CSPs) are highly conserved from bacteria to higher plants and animals. Bacterial cold shock proteins function as RNA chaperones by destabilizing RNA secondary structures and promoting translation as an adaptative mechanism to low temperature stress. In animals, cold shock domain proteins exhibit broad functions related to growth and development. In order to understand better the function of CSPs in planta, detailed analyses were performed for Arabidopsis thaliana CSPs (AtCSPs) on the transcript and protein levels using an extensive series of tissue harvested throughout developmental stages within the entire life cycle of Arabidopsis. On both the transcript and protein levels, AtCSPs were enriched in shoot apical meristems and siliques. Although all AtCSPs exhibited similar expression patterns, AtCSP2 was the most abundantly expressed gene. In situ hybridization analyses were also used to confirm that AtCSP2 and AtCSP4 transcripts accumulate in developing embryos and shoot apices. AtCSPs transcripts were also induced during a controlled floral induction study. In vivo ChIP analysis confirmed that an embryo expressed MADS box transcription factor, AGL15, interacts within two AtCSP promoter regions and alters the respective patterns of AtCSP transcription. Comparative analysis of AtCSP gene expression between Landsberg and Columbia ecotypes confirmed a 1000-fold reduction of AtCSP4 gene expression in the Landsberg background. Analysis of the AtCSP4 genomic locus identified multiple polymorphisms in putative regulatory cis-elements between the two ecotypes. Collectively, these data support the hypothesis that AtCSPs are involved in the transition to flowering and silique development in Arabidopsis.

Original languageEnglish
Pages (from-to)1047-1062
Number of pages16
JournalJournal of Experimental Botany
Volume60
Issue number3
DOIs
StatePublished - Mar 2009

Bibliographical note

Funding Information:
We would like to thank Dr Ryozo Imai and Mr Kentaro Sasaki (National Agricultural Research Center for Hokkaido Region) for kindly providing the anti-WCSP1 antibody. We would also like to thank Dr Joseph Morton (West Virginia University) for graciously supporting our microscopy work. We would like to thank the ABRC for generously providing seeds for the co-2 and ft-2 flowering time mutants. We also thank Dr Donna Fernandez for providing agl15/18 double knock-out and AGL15 overexpression seeds. We thank GABI-KAT for the GK-623B08.01 T-DNA insertion line. This project was supported by a National Science Foundation grant (IBN-0416945) to DK. West Virginia Agriculture and Forestry Experiment Station Scientific Article No. 3028.

Keywords

  • Cold shock domain proteins
  • Floral development
  • RNA binding proteins
  • Silique development

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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