Arsenite induces p70S6K1 activation and HIF-1α expression in prostate cancer cells

Heath D. Skinner, Xiao Song Zhong, Ning Gao, Xianglin Shi, Bing Hua Jiang

Research output: Contribution to journalReview articlepeer-review

25 Scopus citations

Abstract

Arsenite is ubiquitous in the environment, particularly in the form of contaminated water. Although this metal is a known human carcinogen, its exact mechanism of action remains unclear. P70S6K1 phosphorylates the ribosomal 40S protein leading to increased protein translation, and is an important regulator of cell growth and proliferation. Hypoxia inducible factor-1 (HIF1) is a basic helix-loop-helix transcription factor composed of two subunits, HIF-1α and HIF-1β. HIF-1 activates the transcription of a number of genes that mediate angiogenesis and tumor formation. In this study we demonstrated that arsenite treatment increased levelsof p70S6K1 phosphorylation and p70S6K1 activity in a PI3K and mTOR sensitive manner. We have also shown that arsenite specifically induces HIF-1α, but not HIF-1β, protein levels in prostate cancer cells in a mTOR-dependent manner.

Original languageEnglish
Pages (from-to)19-23
Number of pages5
JournalMolecular and Cellular Biochemistry
Volume255
Issue number1-2
DOIs
StatePublished - Jan 2004

Keywords

  • Arsenite
  • HIF-1
  • PI3K
  • mTOR
  • p70S6K1

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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