Arsenite oxidation by Alcaligenes faecalis strain 01201 in a continuous-flow bioreactor

Yi Tin Wang, Arthon Suttigarn

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


As(III) oxidation by a pure bacterial culture, Alcaligenes faecalis strain O1201, was evaluated using a continuous stirred tank reactor. The bioreactor system was operated for 204 days under a wide range of influent As(III) concentrations (500-8,000 mg/L) and hydraulic retention times (25.6-90.7 h) with citrate as the sole carbon and energy source at pH 7 and 30°C. Nine steady-state conditions were obtained with As(III) oxidation, efficiency exceeding 95%. The system quickly recovered from an As(III) overloading at 15,000 mg/day/L while it operated at a HRT of 12.8 h. A material balance analysis revealed, that nearly all the As(III) removed, in the continuous stirred tank reactor (CSTR) was oxidized to As(V) and only 3.2% of the As(III) fed to the CSTR was unaccounted for. Biokinetic parameters, k mc=0.86 mg/mg cell dry weight/h and K c=70 mg/L, for As(III) oxidation were obtained using the Monod expression and data obtained at steady-state conditions.

Original languageEnglish
Pages (from-to)471-476
Number of pages6
JournalJournal of Environmental Engineering
Issue number5
StatePublished - 2007


  • Arsenic
  • Bacteria
  • Kinetics
  • Oxidation
  • Reactors

ASJC Scopus subject areas

  • General Environmental Science
  • Environmental Engineering
  • Environmental Chemistry
  • Civil and Structural Engineering


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