TY - JOUR
T1 - Artefactual low lymphocyte activity caused by platelet contamination in the mononuclear cell preparations
AU - Rueda, F.
AU - Marti, F.
AU - Pinol, G.
AU - Remacha, A.
PY - 1989/6
Y1 - 1989/6
N2 - Platelet (PLT) contamination in preparations of mononuclear cells (MNC) produces a significant decrease (P<0.02) in lymphoproliferative activity and interleukin 2 (IL2) production. This effect was observed in MNC preparations from either healthy blood donors or polyglobulic patients. In a dose‐response curve carried out with increasing PLT number per MNC, progressive inhibition was found, reaching to 62.6% and 49.5%, respectively, to phytohemagglutinin and IL2 lymphoproliferative response and 87.8% in IL2 production, at 80 PLTs/MNC.
AB - Platelet (PLT) contamination in preparations of mononuclear cells (MNC) produces a significant decrease (P<0.02) in lymphoproliferative activity and interleukin 2 (IL2) production. This effect was observed in MNC preparations from either healthy blood donors or polyglobulic patients. In a dose‐response curve carried out with increasing PLT number per MNC, progressive inhibition was found, reaching to 62.6% and 49.5%, respectively, to phytohemagglutinin and IL2 lymphoproliferative response and 87.8% in IL2 production, at 80 PLTs/MNC.
KW - blood platelets
KW - interleukin 2
KW - lymphocytes
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U2 - 10.1002/ajh.2830310210
DO - 10.1002/ajh.2830310210
M3 - Article
C2 - 2786671
AN - SCOPUS:0024405190
SN - 0361-8609
VL - 31
SP - 126
EP - 127
JO - American Journal of Hematology
JF - American Journal of Hematology
IS - 2
ER -