TY - JOUR
T1 - Ascorbic acid is an endogenous cytosolic inhibitor of ATP-supported rat liver mitochondrial calcium transport
AU - Le Vine, H.
AU - Bronson, D. D.
AU - Khouri, R.
AU - Sahyoun, N. E.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1983
Y1 - 1983
N2 - ATP-supported but not Site I or Site II respiratory chain-linked 45Ca2+ transport into isolated rat liver mitochondria is profoundly inhibited by a small molecule present in the cytosolic fraction. This inibitor was purified and shown to be identical with ascorbic acid in a number of chemical properties, cytosolic abundance, susceptibility to ascorbate oxidase, and to agents that otherwise block the effect of authentic ascorbic acid. Experiments with a variety of free radical scavengers and glutathione indicated that ascorbate inhibition of calcium transport is mediated through a 1-electron-free radical mechanism rather than a conventional 2-electron reaction. Calcium transport mechanisms may, therefore, be a target in the pathophysiology of disease processes that influence the intracellular ratios and levels of ascorbate and physiological radical scavengers.
AB - ATP-supported but not Site I or Site II respiratory chain-linked 45Ca2+ transport into isolated rat liver mitochondria is profoundly inhibited by a small molecule present in the cytosolic fraction. This inibitor was purified and shown to be identical with ascorbic acid in a number of chemical properties, cytosolic abundance, susceptibility to ascorbate oxidase, and to agents that otherwise block the effect of authentic ascorbic acid. Experiments with a variety of free radical scavengers and glutathione indicated that ascorbate inhibition of calcium transport is mediated through a 1-electron-free radical mechanism rather than a conventional 2-electron reaction. Calcium transport mechanisms may, therefore, be a target in the pathophysiology of disease processes that influence the intracellular ratios and levels of ascorbate and physiological radical scavengers.
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M3 - Article
C2 - 6654897
AN - SCOPUS:0021071312
SN - 0021-9258
VL - 258
SP - 14954
EP - 14959
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 24
ER -