Abstract
This article describes common laboratory procedures that can reduce the risk of culture contamination, collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a biosafety cabinet. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on petri dishes.
Original language | English |
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Article number | e31 |
Journal | Current Protocols in Essential Laboratory Techniques |
Volume | 18 |
Issue number | 1 |
DOIs | |
State | Published - Jun 2019 |
Bibliographical note
Publisher Copyright:© 2019 John Wiley & Sons, Inc.
Keywords
- biosafety cabinet
- bunsen burner
- laminar flow
- laminar hood
- sterile technique
- sterile work
ASJC Scopus subject areas
- Chemistry (miscellaneous)
- Biochemistry, Genetics and Molecular Biology (miscellaneous)