Aseptic Technique

Tomasz Bykowski; Jonathan F. Holt; Brian Stevenson

doi:10.1002/cpet.31

Aseptic Technique

Tomasz Bykowski, Jonathan F. Holt, Brian Stevenson

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

This article describes common laboratory procedures that can reduce the risk of culture contamination, collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a biosafety cabinet. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on petri dishes.

Original language	English
Article number	e31
Journal	Current Protocols in Essential Laboratory Techniques
Volume	18
Issue number	1
DOIs	https://doi.org/10.1002/cpet.31
State	Published - Jun 2019

Bibliographical note

Publisher Copyright:
© 2019 John Wiley & Sons, Inc.

Keywords

biosafety cabinet
bunsen burner
laminar flow
laminar hood
sterile technique
sterile work

ASJC Scopus subject areas

Chemistry (miscellaneous)
Biochemistry, Genetics and Molecular Biology (miscellaneous)

Access to Document

10.1002/cpet.31

Cite this

@article{63d03a8051f14d479c4b24e93d54f97b,

title = "Aseptic Technique",

abstract = "This article describes common laboratory procedures that can reduce the risk of culture contamination, collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a biosafety cabinet. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on petri dishes.",

keywords = "biosafety cabinet, bunsen burner, laminar flow, laminar hood, sterile technique, sterile work",

author = "Tomasz Bykowski and Holt, {Jonathan F.} and Brian Stevenson",

note = "Publisher Copyright: {\textcopyright} 2019 John Wiley & Sons, Inc.",

year = "2019",

month = jun,

doi = "10.1002/cpet.31",

language = "English",

volume = "18",

number = "1",

}

TY - JOUR

T1 - Aseptic Technique

AU - Bykowski, Tomasz

AU - Holt, Jonathan F.

AU - Stevenson, Brian

PY - 2019/6

Y1 - 2019/6

N2 - This article describes common laboratory procedures that can reduce the risk of culture contamination, collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a biosafety cabinet. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on petri dishes.

AB - This article describes common laboratory procedures that can reduce the risk of culture contamination, collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a biosafety cabinet. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on petri dishes.

KW - biosafety cabinet

KW - bunsen burner

KW - laminar flow

KW - laminar hood

KW - sterile technique

KW - sterile work

UR - http://www.scopus.com/inward/record.url?scp=85064692769&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064692769&partnerID=8YFLogxK

U2 - 10.1002/cpet.31

DO - 10.1002/cpet.31

M3 - Article

AN - SCOPUS:85064692769

VL - 18

JO - Current Protocols in Essential Laboratory Techniques

JF - Current Protocols in Essential Laboratory Techniques

IS - 1

M1 - e31

ER -

Aseptic Technique

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this