Aseptic Technique

Tomasz Bykowski, Jonathan F. Holt, Brian Stevenson

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


This article describes common laboratory procedures that can reduce the risk of culture contamination, collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a biosafety cabinet. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on petri dishes.

Original languageEnglish
Article numbere31
JournalCurrent Protocols in Essential Laboratory Techniques
Issue number1
StatePublished - Jun 2019

Bibliographical note

Publisher Copyright:
© 2019 John Wiley & Sons, Inc.


  • biosafety cabinet
  • bunsen burner
  • laminar flow
  • laminar hood
  • sterile technique
  • sterile work

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)


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