This article describes common laboratory procedures that can reduce the risk of culture contamination, collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a biosafety cabinet. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on petri dishes.
|Journal||Current Protocols in Essential Laboratory Techniques|
|State||Published - Jun 2019|
Bibliographical notePublisher Copyright:
© 2019 John Wiley & Sons, Inc.
Copyright 2019 Elsevier B.V., All rights reserved.
- biosafety cabinet
- bunsen burner
- laminar flow
- laminar hood
- sterile technique
- sterile work
ASJC Scopus subject areas
- Chemistry (miscellaneous)
- Biochemistry, Genetics and Molecular Biology (miscellaneous)