Aseptic Technique

Tomasz Bykowski; Jonathan F. Holt; Brian Stevenson

doi:10.1002/cpet.31

Aseptic Technique

Tomasz Bykowski, Jonathan F. Holt, Brian Stevenson

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

This article describes common laboratory procedures that can reduce the risk of culture contamination, collectively referred as “aseptic technique.” Two major strategies of aseptic work are described: using a Bunsen burner and a biosafety cabinet. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and aliquotting, preparation of growth media, inoculation, passaging, and spreading microorganisms on petri dishes.

Original language	English
Article number	e31
Journal	Current Protocols in Essential Laboratory Techniques
Volume	18
Issue number	1
DOIs	https://doi.org/10.1002/cpet.31
State	Published - Jun 2019

Bibliographical note

Publisher Copyright:
© 2019 John Wiley & Sons, Inc.

Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.

Keywords

biosafety cabinet
bunsen burner
laminar flow
laminar hood
sterile technique
sterile work

ASJC Scopus subject areas

Chemistry (miscellaneous)
Biochemistry, Genetics and Molecular Biology (miscellaneous)

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10.1002/cpet.31

Cite this

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Aseptic Technique

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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