Abstract
This article describes common laboratory procedures that can reduce the risk of culture contamination (sepsis), collectively referred as “aseptic technique.” Two major strategies for aseptic work are described: using a Bunsen burner and using a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and dispensing aliquots, preparing growth media, and inoculating, passaging, and spreading microorganisms on petri dishes.
Original language | English |
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Article number | e98 |
Journal | Current Protocols in Microbiology |
Volume | 56 |
Issue number | 1 |
DOIs | |
State | Published - Feb 1 2020 |
Bibliographical note
Publisher Copyright:© 2020 John Wiley & Sons, Inc.
Keywords
- Bunsen burner
- aseptic technique
- bacteriology
- cell culture
- laminar flow
- laminar hood
- sterilization
- virology
ASJC Scopus subject areas
- Parasitology
- Microbiology
- Virology