Assay for the transbilayer movement of polyisoprenoid-linked saccharides based on the transport of water-soluble analogues

Jeffrey S. Rush; C. J. Waechter

doi:10.1016/j.ymeth.2004.10.002

Assay for the transbilayer movement of polyisoprenoid-linked saccharides based on the transport of water-soluble analogues

Jeffrey S. Rush, C. J. Waechter

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Flippases are a class of membrane proteins that are proposed to facilitate the transbilayer movement of amphipathic polar lipids that are required for membrane biogenesis and the assembly of many diverse complex glycoconjugates in eukaryotic and prokaryotic cells. Despite their crucial roles in membrane biology, very little is known about their structures and the precise mechanism(s) by which they overcome the biophysical barriers of the hydrophobic core, and allow polar head groups to traverse membrane bilayers. This chapter presents methods based on the transport of water-soluble analogues that can be applied to investigate membrane proteins mediating the transverse diffusion of polyisoprenoid-linked glycolipid intermediates involved in the biosynthesis of N-linked glycoproteins, glycosylphosphatidylinositol anchors and bacterial polysaccharides.

Original language	English
Pages (from-to)	316-322
Number of pages	7
Journal	Methods
Volume	35
Issue number	4
DOIs	https://doi.org/10.1016/j.ymeth.2004.10.002
State	Published - Apr 2005

Bibliographical note

Funding Information:
The studies reported by the authors were supported by NIH Grant GM36065 (C.J.W.).

ASJC Scopus subject areas

Molecular Biology
General Biochemistry, Genetics and Molecular Biology

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10.1016/j.ymeth.2004.10.002

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title = "Assay for the transbilayer movement of polyisoprenoid-linked saccharides based on the transport of water-soluble analogues",

abstract = "Flippases are a class of membrane proteins that are proposed to facilitate the transbilayer movement of amphipathic polar lipids that are required for membrane biogenesis and the assembly of many diverse complex glycoconjugates in eukaryotic and prokaryotic cells. Despite their crucial roles in membrane biology, very little is known about their structures and the precise mechanism(s) by which they overcome the biophysical barriers of the hydrophobic core, and allow polar head groups to traverse membrane bilayers. This chapter presents methods based on the transport of water-soluble analogues that can be applied to investigate membrane proteins mediating the transverse diffusion of polyisoprenoid-linked glycolipid intermediates involved in the biosynthesis of N-linked glycoproteins, glycosylphosphatidylinositol anchors and bacterial polysaccharides.",

author = "Rush, {Jeffrey S.} and Waechter, {C. J.}",

note = "Funding Information: The studies reported by the authors were supported by NIH Grant GM36065 (C.J.W.).",

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T1 - Assay for the transbilayer movement of polyisoprenoid-linked saccharides based on the transport of water-soluble analogues

AU - Rush, Jeffrey S.

AU - Waechter, C. J.

N1 - Funding Information: The studies reported by the authors were supported by NIH Grant GM36065 (C.J.W.).

PY - 2005/4

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AB - Flippases are a class of membrane proteins that are proposed to facilitate the transbilayer movement of amphipathic polar lipids that are required for membrane biogenesis and the assembly of many diverse complex glycoconjugates in eukaryotic and prokaryotic cells. Despite their crucial roles in membrane biology, very little is known about their structures and the precise mechanism(s) by which they overcome the biophysical barriers of the hydrophobic core, and allow polar head groups to traverse membrane bilayers. This chapter presents methods based on the transport of water-soluble analogues that can be applied to investigate membrane proteins mediating the transverse diffusion of polyisoprenoid-linked glycolipid intermediates involved in the biosynthesis of N-linked glycoproteins, glycosylphosphatidylinositol anchors and bacterial polysaccharides.

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JO - Methods

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Assay for the transbilayer movement of polyisoprenoid-linked saccharides based on the transport of water-soluble analogues

Abstract

Bibliographical note

ASJC Scopus subject areas

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