Assembly of bacteriophage T4 tail fibers. IV. Subunit composition of tail fibers and fiber precursors

Robert C. Dickson

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Using a novel purification procedure, the protein composition of the tail fibers of bacteriophage T4 has been determined. Fibers contain four proteins whose molecular weights, as estimated by sodium dodecyl sulfate/acrylamide gel electrophoresis, are 150,000, 125,000, 40,000 and 24,000. The two largest proteins have been previously identified as the products of genes 34 (P34) and 37 (P37), respectively (King and Laemmli, 1971; Ward and Dickson, 1971). The two smaller proteins have now been identified as the products of genes 35 (P35) and 36 (P36), respectively. The products of the two other known phage genes required for fiber assembly, 38 and 57, have been identified as non-structural phage proteins with molecular weights of 26,000 and 10,000, respectively.

Original languageEnglish
Pages (from-to)633-638,IN1-IN7,639-647
JournalJournal of Molecular Biology
Volume79
Issue number4
DOIs
StatePublished - Oct 5 1973

Bibliographical note

Funding Information:
I thank Dr William B. Wood for his hospitality, advice and criticism during this research and for critically reviewing this manuscript. Grant no. AI09238 from the United States Public Health Service to Dr Wood supported this research. I was supported by a postdoctoral fellowship GM49918 from the United States Public Health Service.

Funding

I thank Dr William B. Wood for his hospitality, advice and criticism during this research and for critically reviewing this manuscript. Grant no. AI09238 from the United States Public Health Service to Dr Wood supported this research. I was supported by a postdoctoral fellowship GM49918 from the United States Public Health Service.

FundersFunder number
U.S. Public Health ServiceGM49918
U.S. Public Health Service

    ASJC Scopus subject areas

    • Molecular Biology
    • Biophysics
    • Structural Biology

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