Assessing the biological activity of the glucan phosphatase laforin

Carlos Romá-Mateo, Madushi Raththagala, Mathew S. Gentry, Pascual Sanz

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Glucan phosphatases are a recently discovered family of enzymes that dephosphorylate either starch or glycogen and are essential for proper starch metabolism in plants and glycogen metabolism in humans. Mutations in the gene encoding the only human glucan phosphatase, laforin, result in the fatal, neurodegenerative, epilepsy known as Lafora disease. Here, we describe phosphatase assays to assess both generic laforin phosphatase activity and laforin’s unique glycogen phosphatase activity.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages13
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.


  • Dual-specificity phosphatase
  • Glycogen
  • Lafora disease

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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