Assessing the biological activity of the glucan phosphatase laforin

Carlos Romá-Mateo; Madushi Raththagala; Mathew S. Gentry; Pascual Sanz

doi:10.1007/978-1-4939-3746-2_7

Assessing the biological activity of the glucan phosphatase laforin

Carlos Romá-Mateo, Madushi Raththagala, Mathew S. Gentry, Pascual Sanz

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Glucan phosphatases are a recently discovered family of enzymes that dephosphorylate either starch or glycogen and are essential for proper starch metabolism in plants and glycogen metabolism in humans. Mutations in the gene encoding the only human glucan phosphatase, laforin, result in the fatal, neurodegenerative, epilepsy known as Lafora disease. Here, we describe phosphatase assays to assess both generic laforin phosphatase activity and laforin’s unique glycogen phosphatase activity.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	107-119
Number of pages	13
DOIs	https://doi.org/10.1007/978-1-4939-3746-2_7
State	Published - 2016

Publication series

Name	Methods in Molecular Biology
Volume	1447
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

Keywords

Dual-specificity phosphatase
Glycogen
Lafora disease

ASJC Scopus subject areas

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-3746-2_7

Cite this

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AU - Sanz, Pascual

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AB - Glucan phosphatases are a recently discovered family of enzymes that dephosphorylate either starch or glycogen and are essential for proper starch metabolism in plants and glycogen metabolism in humans. Mutations in the gene encoding the only human glucan phosphatase, laforin, result in the fatal, neurodegenerative, epilepsy known as Lafora disease. Here, we describe phosphatase assays to assess both generic laforin phosphatase activity and laforin’s unique glycogen phosphatase activity.

KW - Dual-specificity phosphatase

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Assessing the biological activity of the glucan phosphatase laforin

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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