Abstract
Glucan phosphatases are a recently discovered family of enzymes that dephosphorylate either starch or glycogen and are essential for proper starch metabolism in plants and glycogen metabolism in humans. Mutations in the gene encoding the only human glucan phosphatase, laforin, result in the fatal, neurodegenerative, epilepsy known as Lafora disease. Here, we describe phosphatase assays to assess both generic laforin phosphatase activity and laforin’s unique glycogen phosphatase activity.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Pages | 107-119 |
| Number of pages | 13 |
| DOIs | |
| State | Published - 2016 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1447 |
| ISSN (Print) | 1064-3745 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media New York 2016.
Funding
This study was supported by grants from the Spanish Ministry of Education and Science SAF2014-54604-C3-1-R (P.S.), Generalitat Valenciana (PrometeoII/2014/029) (P.S.), NIH Grants R01NS070899 (M.S.G.), P20GM103486 (M.S.G.), Mizutani Foundation for Glycoscience Award (M.S.G.), and NSF Grant IIA-1355438 (M.S.G.). C.R.-M. was supported by the Saving Lives at Birth Consortium.
| Funders | Funder number |
|---|---|
| National Science Foundation (NSF) | IIA-1355438 |
| National Institutes of Health (NIH) | R01NS070899 |
| National Institute of General Medical Sciences | P20GM103486 |
| Mizutani Foundation for Glycoscience | |
| Ministerio de Educación, Cultura y Deporte | SAF2014-54604-C3-1-R |
| Generalitat Valenciana | PrometeoII/2014/029 |
Keywords
- Dual-specificity phosphatase
- Glycogen
- Lafora disease
ASJC Scopus subject areas
- Molecular Biology
- Genetics
