Assessment of correlation between in vitro CD3 + T cell susceptibility to EAV infection and clinical outcome following experimental infection

Yun Young Go, R. Frank Cook, Juliana Q. Fulgêncio, Juliana R. Campos, Pamela Henney, Peter J. Timoney, David W. Horohov, Udeni B.R. Balasuriya

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


In a recent study, we demonstrated that the virulent Bucyrus strain (VBS) of EAV could infect in vitro a small population of CD3 + T lymphocytes from some but not all horses. Furthermore, we have shown that a common haplotype is associated with this in vitro CD3 + T cell susceptibility/resistance phenotype to EAV infection. In this study, we investigated whether the differences in the susceptibility or resistance of CD3 + T cells in vitro correlate with the outcome and severity of clinical signs in vivo. Thus, horses were divided into two groups based on their CD3 + T cell susceptible or resistant phenotype. Following experimental inoculation with the recombinant VBS of EAV, horses were assessed for presence and severity of clinical signs, duration and magnitude of virus shedding, as well as production of proinflammatory and immunomodulatory cytokines in peripheral blood mononuclear cells using real-time quantitative RT-PCR. The data showed that there was a significant difference between the two groups of horses in terms of cytokine mRNA expression and evidence of increased clinical signs in horses possessing the in vitro CD3 + T cell resistant phenotype. This is the first study to provide direct evidence for a correlation between variation in host genotype and phenotypic differences in terms of the extent of viral replication, presence and severity of clinical signs and cytokine gene expression caused by infection with virulent EAV.

Original languageEnglish
Pages (from-to)220-225
Number of pages6
JournalVeterinary Microbiology
Issue number1-2
StatePublished - May 25 2012

Bibliographical note

Funding Information:
The authors would like to thank the staff at the University of Kentucky's Maine Chance Farm for their excellent care of the horses. In addition, the authors are grateful to Dr. Ernest Bailey, University of Kentucky, for critical reading of the manuscript. This work was supported by the Frederick Van Lennep Chair endowment fund at the Maxwell H. Gluck Equine Research Center, University of Kentucky . Yun Young Go is supported by a Geoffrey C. Hughes Foundation graduate fellowship.


  • CD3 T lymphocytes
  • Cytokines
  • Equine arteritis virus
  • Equine viral arteritis
  • Genetic susceptibility

ASJC Scopus subject areas

  • Microbiology
  • General Veterinary


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