Association of L-type calcium channels with a vacuolar H+-ATPase G2 subunit

Tianyan Gao, M. Marlene Hosey

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The class C L-type calcium (Ca2+) channels have been implicated in many important physiological processes. Here, we have identified a mouse vacuolar H+-ATPase (V-ATPase) G2 subunit protein that bound to the C-terminal domain of the pore-forming α(1C) subunit using a yeast two-hybrid screen. Protein-protein interaction between the V-ATPase G subunit and the α(1C) subunit was confirmed using in vitro GST pull-down assays and coimmunoprecipitation from intact cells. Moreover, treatment of cells expressing L-type Ca2+ channels with a specific inhibitor of the V-ATPase blocked proper targeting of the channels to the plasma membrane. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)611-616
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Nov 2 2000

Bibliographical note

Funding Information:
This work was supported by NIH Grant R37-23306 to M.M.H. We thank Dr. Heidi Hamm and E. J. Dell for their help with yeast two-hybrid screening.


  • L-type calcium channel
  • Membrane targeting
  • Protein-protein interaction
  • Vacuolar H-ATPase G subunit
  • Yeast two-hybrid screening

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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