Ataxin-10 interacts with O-GlcNAc transferase OGT in pancreatic β cells

Sreenath S. Andrali; Pia März; Sabire Özcan

doi:10.1016/j.bbrc.2005.09.026

Ataxin-10 interacts with O-GlcNAc transferase OGT in pancreatic β cells

Sreenath S. Andrali
, Pia März
, Sabire Özcan

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Several nuclear and cytoplasmic proteins in metazoans are modified by O-linked N-acetylglucosamine (O-GlcNAc). This modification is dynamic and reversible similar to phosphorylation and is catalyzed by the O-linked GlcNAc transferase (OGT). Hyperglycemia has been shown to increase O-GlcNAc levels in pancreatic β cells, which appears to interfere with β-cell function. To obtain a better understanding of the role of O-linked GlcNAc modification in β cells, we have isolated OGT interacting proteins from a cDNA library made from the mouse insulinoma MIN6 cell line. We describe here the identification of Ataxin-10, encoded by the SCA10 (spinocerebellar ataxia type 10) gene as an OGT interacting protein. Mutations in the SCA10 gene cause progressive cerebellar ataxias and seizures. We demonstrate that SCA10 interacts with OGT in vivo and is modified by O-linked glycosylation in MIN6 cells, suggesting a novel role for the Ataxin-10 protein in pancreatic β cells.

Original language	English
Pages (from-to)	149-153
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	337
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.09.026
State	Published - Nov 11 2005

Bibliographical note

Funding Information:
We thank Jessica Curry and John Hoben for their help with the Cytotrap screen, John Hanover (NIH) for the human cDNA clone. This work was supported by a grant from NIH (R21DK065730 to S.Ö) and a Postdoctoral fellowship from the American Heart Association, Ohio Valley, to S.S.A.

Funding

We thank Jessica Curry and John Hoben for their help with the Cytotrap screen, John Hanover (NIH) for the human cDNA clone. This work was supported by a grant from NIH (R21DK065730 to S.Ö) and a Postdoctoral fellowship from the American Heart Association, Ohio Valley, to S.S.A.

Funders	Funder number
Ohio Valley Medical Center
National Institutes of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases	R21DK065730
American Heart Association

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Ataxia
Ataxin-10
Diabetes
Glucose
MIN6
O-GlcNAc
OGT
SCA10
β cells

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2005.09.026

Cite this

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title = "Ataxin-10 interacts with O-GlcNAc transferase OGT in pancreatic β cells",

abstract = "Several nuclear and cytoplasmic proteins in metazoans are modified by O-linked N-acetylglucosamine (O-GlcNAc). This modification is dynamic and reversible similar to phosphorylation and is catalyzed by the O-linked GlcNAc transferase (OGT). Hyperglycemia has been shown to increase O-GlcNAc levels in pancreatic β cells, which appears to interfere with β-cell function. To obtain a better understanding of the role of O-linked GlcNAc modification in β cells, we have isolated OGT interacting proteins from a cDNA library made from the mouse insulinoma MIN6 cell line. We describe here the identification of Ataxin-10, encoded by the SCA10 (spinocerebellar ataxia type 10) gene as an OGT interacting protein. Mutations in the SCA10 gene cause progressive cerebellar ataxias and seizures. We demonstrate that SCA10 interacts with OGT in vivo and is modified by O-linked glycosylation in MIN6 cells, suggesting a novel role for the Ataxin-10 protein in pancreatic β cells.",

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note = "Funding Information: We thank Jessica Curry and John Hoben for their help with the Cytotrap screen, John Hanover (NIH) for the human cDNA clone. This work was supported by a grant from NIH (R21DK065730 to S.{\"O}) and a Postdoctoral fellowship from the American Heart Association, Ohio Valley, to S.S.A.",

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AU - Özcan, Sabire

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Ataxin-10 interacts with O-GlcNAc transferase OGT in pancreatic β cells

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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