Attenuation of acute mitochondrial dysfunction after traumatic brain injury in mice by NIM811, a non-immunosuppressive cyclosporin A analog

L. H. Mbye, I. N. Singh, P. G. Sullivan, J. E. Springer, E. D. Hall

Research output: Contribution to journalArticlepeer-review

96 Scopus citations

Abstract

Following traumatic brain injury (TBI), mitochondrial function becomes compromised. Mitochondrial dysfunction is characterized by intra-mitochondrial Ca2+ accumulation, induction of oxidative damage, and mitochondrial permeability transition (mPT). Experimental studies show that cyclosporin A (CsA) inhibits mPT. However, CsA also inhibits calcineurin. In the present study, we conducted a dose-response analysis of NIM811, a non-calcineurin inhibitory CsA analog, on mitochondrial dysfunction following TBI in mice, and compared the effects of the optimal dose of NIM811 (10 mg/kg i.p.) against an optimized dose of CsA (20 mg/kg i.p.). Male CF-1 mice were subjected to severe TBI utilizing the controlled cortical impact model. Mitochondrial respiration was assessed from animals treated with either NIM811, CsA, or vehicle 15 min post-injury. The respiratory control ratio (RCR) of mitochondria from vehicle-treated animals was significantly (p < 0.01) lower at 3 or 12 h post-TBI, relative to shams. Treatment of animals with either NIM811 or CsA significantly (p < 0.03) attenuated this reduction. Consistent with this finding, both NIM811 and CsA significantly reduced lipid peroxidative and protein nitrative damage to mitochondria at 12 h post-TBI. These results showing the ability of NIM811 to fully duplicate the mitochondrial protective efficacy of CsA supports the conclusion that inhibition of the mPT may be sufficient to explain CsA's protective effects.

Original languageEnglish
Pages (from-to)243-253
Number of pages11
JournalExperimental Neurology
Volume209
Issue number1
DOIs
StatePublished - Jan 2008

Bibliographical note

Funding Information:
This research was supported by funding from the NIH NS 1R01 NS46566 (EDH) and NS048191 (PGS) and the Kentucky Spinal Cord and Head Injury Research Trust (KSCHIRT) (EDH). The authors also thank Kimberly M. Carrico for expert technical assistance.

Keywords

  • Cyclosporin A
  • Mitochondrial permeability transition
  • NIM811
  • Oxidative damage
  • Respiratory control ratio
  • Traumatic brain injury

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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