Using a variety of techniques, including limiting dilution assays (LDA), intracellular IFNγ assays, and Db-IgG1 MHC dimer staining to measure viral peptide-specific T cell number and function, we show here that heterologous virus infections quantitatively delete and qualitatively alter the memory pool of T cells specific to a previously encountered virus. We also show that a prior history of a virus infection can alter the hierarchy of the immunodominant peptide response to a second virus and that virus infections selectively reactivate memory T cells with distinct specificities to earlier viruses. These results are consistent with a model for the immune system that accommodates memory T cell populations for multiple pathogens over the course of a lifetime.
|Number of pages||10|
|State||Published - Dec 1999|
Bibliographical noteFunding Information:
We thank Dr. M. B. A. Oldstone for LCMV variants and I. Stroynowski and G. Haemmerling for their technical assistance. This work was supported by National Institutes of Health research grants AR-35506 and AI-17672 to R. M. W.; Clinical Investigator Award AI01362 to L. K. S.; training grants AI-07272 and AI-07349 to L. K. S.; AI-29575, AI-45208, and NMSS (RG 2637A2/1) to J. S.; and CA86030–01 to D. P. The contents of this publication are solely the responsibility of the authors and do not represent the official view of the National Institute of Health.
ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases