Aurora B Kinase-Dependent Recruitment of hZW10 and hROD to Tensionless Kinetochores

Jakub K. Famulski, Gordon K. Chan

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


The mitotic checkpoint ensures proper chromosome segregation by monitoring two critical events during mitosis. One is kinetochore attachment to the mitotic spindle, and the second is the alignment of chromosomes at the metaphase plate, resulting in tension across sister kinetochores (reviewed in [1, 2]). Mitotic-checkpoint proteins are known to accumulate at unaligned chromosomes that have not achieved proper kinetochore-microtubule attachments or established an adequate level of tension across sister kinetochores [3]. Here, we report that hZW10 and hROD, two components of the evolutionarily conserved RZZ complex [4, 5], accumulate at kinetochores in response to the loss of tension. By using live-cell imaging and FRAP, we showed that the accumulation of hZW10 at tensionless kinetochores stems from a 4-fold reduction of kinetochore turnover rate. We also found that cells lacking hZW10 escape loss-of-tension-induced mitotic-checkpoint arrest more rapidly than those arrested in response to the lack of kinetochore-microtubule attachments. Furthermore, we show that pharmacological inhibition of Aurora B kinase activity with ZM447439 in the absence of tension, but not in the absence of kinetochore-microtubule attachments, results in the loss of hZW10, hROD, and hBub1 from kinetochores. We therefore conclude that Aurora B kinase activity is required for the accumulation of tension-sensitive mitotic-checkpoint components, such as hZW10 and hROD, in order to maintain mitotic-checkpoint arrest.

Original languageEnglish
Pages (from-to)2143-2149
Number of pages7
JournalCurrent Biology
Issue number24
StatePublished - Dec 18 2007



ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences


Dive into the research topics of 'Aurora B Kinase-Dependent Recruitment of hZW10 and hROD to Tensionless Kinetochores'. Together they form a unique fingerprint.

Cite this