Autohydrolysis pretreatment of Coastal Bermuda grass for increased enzyme hydrolysis

Jung Myoung Lee, Jian Shi, Richard A. Venditti, Hasan Jameel

Research output: Contribution to journalArticlepeer-review

107 Scopus citations


Coastal Bermuda grass (GBG) was pretreated using an autohydrolysis process with different temperatures and times, and the pretreated materials were enzymatically hydrolyzed using a mixture of cellulase, xylanase and β-glucosidase with different enzyme loadings to evaluate sugar yields. Compared with untreated CBG, autohydrolysis pretreatments at all elevated temperatures and residence times tested enhanced enzymatic digestibility of both cellulose and hemicellulose. Increasing the temperature and residence time also helps to solubilize hemicelluloses, with 83.3% of the hemicelluloses solubilized at 170 °C for 60 min treatment. However, higher temperatures and longer times resulted in an overall lower sugar recovery when considering monosaccharides in the prehydrolyzate combined with the enzyme hydrolyzate. Autohydrolysis at 150 °C for 60 min provided the highest overall sugar yield for the entire process. A total of 43.3 g of sugars, 70% of the theoretical sugar yield, can be generated from 100 g CBG, 15.0 g of monosaccharide in the prehydrolyzate and 28.3 g in the enzyme hydrolyzate. The conversion efficiency could be further improved by optimizing enzyme dosages and xylanases:cellulases ratio and pretreatment conditions to minimize sugar degradation.

Original languageEnglish
Pages (from-to)6434-6441
Number of pages8
JournalBioresource Technology
Issue number24
StatePublished - Dec 2009

Bibliographical note

Funding Information:
The authors would like to thank North Carolina Biotech Center for funding this collaborative research project between Dr. Jay J. Cheng of the Biological and Agricultural Engineering Department and the authors of this manuscript in the Wood and Paper Science Department at North Carolina State University, NCBC Grant # 2007-MRG-1106. Also thanks to Novozymes Inc for providing enzyme samples.


  • Autohydrolysis
  • Coastal Bermuda grass
  • Enzymatic hydrolysis
  • Sugar analysis

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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