TY - JOUR
T1 - Automated high-throughput system to fractionate plant natural products for drug discovery
AU - Tu, Ying
AU - Jeffries, Cynthia
AU - Ruan, Hong
AU - Nelson, Cynthia
AU - Smithson, David
AU - Shelat, Anang A.
AU - Brown, Kristin M.
AU - Li, Xing Cong
AU - Hester, John P.
AU - Smillie, Troy
AU - Khan, Ikhlas A.
AU - Walker, Larry
AU - Guy, Kip
AU - Yan, Bing
PY - 2010/4/23
Y1 - 2010/4/23
N2 - The development of an automated, high-throughput fractionation procedure to prepare and analyze natural product libraries for drug discovery screening is described. Natural products obtained from plant materials worldwide were extracted and first prefractionated on polyamide solid-phase extraction cartridges to remove polyphenols, followed by high-throughput automated fractionation, drying, weighing, and reformatting for screening and storage. The analysis of fractions with UPLC coupled with MS, PDA, and ELSD detectors provides information that facilitates characterization of compounds in active fractions. Screening of a portion of fractions yielded multiple assay-specific hits in several high-throughput cellular screening assays. This procedure modernizes the traditional natural product fractionation paradigm by seamlessly integrating automation, informatics, and multimodal analytical interrogation capabilities.
AB - The development of an automated, high-throughput fractionation procedure to prepare and analyze natural product libraries for drug discovery screening is described. Natural products obtained from plant materials worldwide were extracted and first prefractionated on polyamide solid-phase extraction cartridges to remove polyphenols, followed by high-throughput automated fractionation, drying, weighing, and reformatting for screening and storage. The analysis of fractions with UPLC coupled with MS, PDA, and ELSD detectors provides information that facilitates characterization of compounds in active fractions. Screening of a portion of fractions yielded multiple assay-specific hits in several high-throughput cellular screening assays. This procedure modernizes the traditional natural product fractionation paradigm by seamlessly integrating automation, informatics, and multimodal analytical interrogation capabilities.
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U2 - 10.1021/np9007359
DO - 10.1021/np9007359
M3 - Article
C2 - 20232897
AN - SCOPUS:77951566660
SN - 0163-3864
VL - 73
SP - 751
EP - 754
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 4
ER -