Automated Operation of Immiscible Filtration Assisted by Surface Tension (IFAST) Arrays for Streamlined Analyte Isolation

Scott M. Berry, Keil J. Regehr, Benjamin P. Casavant, David J. Beebe

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The purification of analytes is an important prerequisite for many analytical processes. Although automated infrastructure has dramatically increased throughput for many of these processes, the upstream analyte purification throughput has lagged behind, partially due to the complexity of conventional isolation processes. Here, we demonstrate automated operation of arrays of a new sample preparation technology-immiscible filtration assisted by surface tension (IFAST). IFAST uses surface tension to position an immiscible liquid barrier between a biological sample and downstream buffer. Paramagnetic particles are used to capture analytes of interest and draw them across the immiscible barrier, thus resulting in purification in a single step. Furthermore, the planarity of the IFAST design enables facile and simultaneous operation of multiple IFAST devices. To demonstrate the application of automation to IFAST, we successfully perform an array of 48 IFAST-based assays to detect the presence of a specific antibody. This assay array uses only a commercial automated liquid handler to load the devices and a custom-built magnet actuator to operate the assays. Automated operation of the IFAST devices resulted in more repeatable results relative to manual operation.

Original languageEnglish
Pages (from-to)206-211
Number of pages6
JournalJournal of laboratory automation
Volume18
Issue number3
DOIs
StatePublished - Jul 2013

Bibliographical note

Funding Information:
The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Funded by a grant from the Bill & Melinda Gates Foundation through the Grand Challenges in Global Health initiative, a DOD PCRP Idea grant (W81XWH-09-1-0192), and an NIH NCI R33 grant (CA137673).

Keywords

  • high throughput
  • immiscible phase
  • microfluidics
  • purification
  • sample preparation

ASJC Scopus subject areas

  • Computer Science Applications
  • Medical Laboratory Technology

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