Automated protein resonance assignments of magic angle spinning solid-state NMR spectra of β1 immunoglobulin binding domain of protein G (GB1)

Hunter N.B. Moseley, Lindsay J. Sperling, Chad M. Rienstra

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Magic-angle spinning solid-state NMR (MAS SSNMR) represents a fast developing experimental technique with great potential to provide structural and dynamics information for proteins not amenable to other methods. However, few automated analysis tools are currently available for MAS SSNMR. We present a methodology for automating protein resonance assignments of MAS SSNMR spectral data and its application to experimental peak lists of the β1 immunoglobulin binding domain of protein G (GB1) derived from a uniformly 13C- and 15N-labeled sample. This application to the 56 amino acid GB1 produced an overall 84.1% assignment of the N, CO, CA, and CB resonances with no errors using peak lists from NCACX 3D, CANcoCA 3D, and CANCOCX 4D experiments. This proof of concept demonstrates the tractability of this problem.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalJournal of Biomolecular NMR
Volume48
Issue number3
DOIs
StatePublished - Nov 2010

Bibliographical note

Funding Information:
Acknowledgments We would like to acknowledge Dr. David A. Snyder’s help during the evolution of the peak list alignment algorithm. This work was supported in part by DOE DE-EM0000197 (to H.M.) and NIH R01-GM075937 (to C.M.R.).

Keywords

  • Automated resonance assignments
  • GB1
  • Magic angle spinning
  • Protein
  • Solid-state

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

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