Autophagy in platelets

Meenakshi Banerjee, Yunjie Huang, Madhu M. Ouseph, Smita Joshi, Irina Pokrovskaya, Brian Storrie, Jinchao Zhang, Sidney W. Whiteheart, Qing Jun Wang

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

11 Scopus citations

Abstract

Anucleate platelets are produced by fragmentation of megakaryocytes. Platelets circulate in the bloodstream for a finite period: upon vessel injury, they are activated to participate in hemostasis; upon senescence, unused platelets are cleared. Platelet hypofunction leads to bleeding. Conversely, pathogenic platelet activation leads to occlusive events that precipitate strokes and heart attacks. Recently, we and others have shown that autophagy occurs in platelets and is important for platelet production and normal functions including hemostasis and thrombosis. Due to the unique properties of platelets, such as their lack of nuclei and their propensity for activation, methods for studying platelet autophagy must be specifically tailored. Here, we describe useful methods for examining autophagy in both human and mouse platelets.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages511-528
Number of pages18
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1880
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

Keywords

  • Autophagy
  • Electron microscopy
  • Hemostasis
  • Live imaging
  • Platelets

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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