Auxin-regulated changes of fatty acid content and composition in soybean zygotic embryo cotyledons

Wennuan Liu; David F. Hildebrand; Glenn B. Collins

doi:10.1016/0168-9452(95)04067-5

Auxin-regulated changes of fatty acid content and composition in soybean zygotic embryo cotyledons

Wennuan Liu, David F. Hildebrand, Glenn B. Collins

Plant and Soil Sciences

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The present studies were conducted to investigate the effect of exogenous auxins on zygotic embryo fatty acid content and lipid composition during somatic embryo induction. Immature soybean (Glycine max (L.) Merr.) cotyledons from two developmental stages were cultured on 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA). The presence of 2,4-D or NAA in the medium altered the content and percentage of oleic (18:1), linoleic (18:2) and linolenic (18:3) acids, with less effects on stearic (18:0), palmitic (16:0) or total fatty acids in the embryonic cotyledons. Desaturation assays with radiolabelled fatty acids showed that 2,4-D increased both the ratios of 18:2 to 18:1 and 18:3 to 18:2. NAA at 10 mg/l resulted in an increase in the content and percentage of 18:3 but not that of 18:2 in the embryonic cotyledons. Analysis of free fatty acids, triglycerides, phosphatidylcholine, phosphatidylethanolamine, and glycolipids (monogalactosyldiacylglycerol and digalactosyldiacylglycerol) revealed that the increased ratio of 18:3 to 18:2 occurred in phosphatidylcholine, triglycerides and free fatty acids, but not in glycolipids. Our data suggest that 2,4-D increased the efficiency of the desaturation related processes in extra plastidial compartments.

Original language	English
Pages (from-to)	31-42
Number of pages	12
Journal	Plant Science
Volume	106
Issue number	1
DOIs	https://doi.org/10.1016/0168-9452(95)04067-5
State	Published - Mar 1995

Bibliographical note

Funding Information:
The researchr eportedi n this paper was sup-portedb y the Kentucky Agricultural Experiment Station, the American SoybeanA ssociationa nd the National Science Foundation, Grant DIR 9114968W. e would like to thank Carl Redmond for usefulc ommentsa nd technicala ssistance.

Funding

The researchr eportedi n this paper was sup-portedb y the Kentucky Agricultural Experiment Station, the American SoybeanA ssociationa nd the National Science Foundation, Grant DIR 9114968W. e would like to thank Carl Redmond for usefulc ommentsa nd technicala ssistance.

Funders	Funder number
American SoybeanA ssociationa
National Science Foundation (NSF)	DIR 9114968W
Kentucky Agricultural Experiment Station

Keywords

Auxin
Embryo
Fatty acids
Glycine max
Lipids

ASJC Scopus subject areas

Genetics
Agronomy and Crop Science
Plant Science

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10.1016/0168-9452(95)04067-5

Cite this

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title = "Auxin-regulated changes of fatty acid content and composition in soybean zygotic embryo cotyledons",

abstract = "The present studies were conducted to investigate the effect of exogenous auxins on zygotic embryo fatty acid content and lipid composition during somatic embryo induction. Immature soybean (Glycine max (L.) Merr.) cotyledons from two developmental stages were cultured on 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA). The presence of 2,4-D or NAA in the medium altered the content and percentage of oleic (18:1), linoleic (18:2) and linolenic (18:3) acids, with less effects on stearic (18:0), palmitic (16:0) or total fatty acids in the embryonic cotyledons. Desaturation assays with radiolabelled fatty acids showed that 2,4-D increased both the ratios of 18:2 to 18:1 and 18:3 to 18:2. NAA at 10 mg/l resulted in an increase in the content and percentage of 18:3 but not that of 18:2 in the embryonic cotyledons. Analysis of free fatty acids, triglycerides, phosphatidylcholine, phosphatidylethanolamine, and glycolipids (monogalactosyldiacylglycerol and digalactosyldiacylglycerol) revealed that the increased ratio of 18:3 to 18:2 occurred in phosphatidylcholine, triglycerides and free fatty acids, but not in glycolipids. Our data suggest that 2,4-D increased the efficiency of the desaturation related processes in extra plastidial compartments.",

keywords = "Auxin, Embryo, Fatty acids, Glycine max, Lipids",

author = "Wennuan Liu and Hildebrand, {David F.} and Collins, {Glenn B.}",

note = "Funding Information: The researchr eportedi n this paper was sup-portedb y the Kentucky Agricultural Experiment Station, the American SoybeanA ssociationa nd the National Science Foundation, Grant DIR 9114968W. e would like to thank Carl Redmond for usefulc ommentsa nd technicala ssistance.",

year = "1995",

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language = "English",

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T1 - Auxin-regulated changes of fatty acid content and composition in soybean zygotic embryo cotyledons

AU - Liu, Wennuan

AU - Hildebrand, David F.

AU - Collins, Glenn B.

N1 - Funding Information: The researchr eportedi n this paper was sup-portedb y the Kentucky Agricultural Experiment Station, the American SoybeanA ssociationa nd the National Science Foundation, Grant DIR 9114968W. e would like to thank Carl Redmond for usefulc ommentsa nd technicala ssistance.

PY - 1995/3

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N2 - The present studies were conducted to investigate the effect of exogenous auxins on zygotic embryo fatty acid content and lipid composition during somatic embryo induction. Immature soybean (Glycine max (L.) Merr.) cotyledons from two developmental stages were cultured on 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA). The presence of 2,4-D or NAA in the medium altered the content and percentage of oleic (18:1), linoleic (18:2) and linolenic (18:3) acids, with less effects on stearic (18:0), palmitic (16:0) or total fatty acids in the embryonic cotyledons. Desaturation assays with radiolabelled fatty acids showed that 2,4-D increased both the ratios of 18:2 to 18:1 and 18:3 to 18:2. NAA at 10 mg/l resulted in an increase in the content and percentage of 18:3 but not that of 18:2 in the embryonic cotyledons. Analysis of free fatty acids, triglycerides, phosphatidylcholine, phosphatidylethanolamine, and glycolipids (monogalactosyldiacylglycerol and digalactosyldiacylglycerol) revealed that the increased ratio of 18:3 to 18:2 occurred in phosphatidylcholine, triglycerides and free fatty acids, but not in glycolipids. Our data suggest that 2,4-D increased the efficiency of the desaturation related processes in extra plastidial compartments.

AB - The present studies were conducted to investigate the effect of exogenous auxins on zygotic embryo fatty acid content and lipid composition during somatic embryo induction. Immature soybean (Glycine max (L.) Merr.) cotyledons from two developmental stages were cultured on 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA). The presence of 2,4-D or NAA in the medium altered the content and percentage of oleic (18:1), linoleic (18:2) and linolenic (18:3) acids, with less effects on stearic (18:0), palmitic (16:0) or total fatty acids in the embryonic cotyledons. Desaturation assays with radiolabelled fatty acids showed that 2,4-D increased both the ratios of 18:2 to 18:1 and 18:3 to 18:2. NAA at 10 mg/l resulted in an increase in the content and percentage of 18:3 but not that of 18:2 in the embryonic cotyledons. Analysis of free fatty acids, triglycerides, phosphatidylcholine, phosphatidylethanolamine, and glycolipids (monogalactosyldiacylglycerol and digalactosyldiacylglycerol) revealed that the increased ratio of 18:3 to 18:2 occurred in phosphatidylcholine, triglycerides and free fatty acids, but not in glycolipids. Our data suggest that 2,4-D increased the efficiency of the desaturation related processes in extra plastidial compartments.

KW - Auxin

KW - Embryo

KW - Fatty acids

KW - Glycine max

KW - Lipids

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AN - SCOPUS:0029137667

SN - 0168-9452

VL - 106

SP - 31

EP - 42

JO - Plant Science

JF - Plant Science

IS - 1

ER -

Auxin-regulated changes of fatty acid content and composition in soybean zygotic embryo cotyledons

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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