Axon contact-driven Schwann cell dedifferentiation

Jennifer Soto, Paula V. Monje

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Mature Schwann cells (SCs) retain dedifferentiation potential throughout adulthood. Still, how dedifferentiation occurs remains uncertain. Results from a variety of cell-based assays using in vitro cultured cAMP-differentiated and myelinating SCs revealed the existence of a novel dedifferentiating activity expressed on the surface of dorsal root ganglion (DRG) axons. This activity had the capacity to prevent SC differentiation and elicit dedifferentiation through direct SC-axon contact. Evidence is provided showing that a rapid loss of myelinating SC markers concomitant to proliferation occurred even in the presence of elevated cAMP, a signal that is required to drive and maintain a differentiated state. The dedifferentiating activity was a membrane-bound protein found exclusively in DRG neurons, as judged by its subcellular partitioning, sensitivity to proteolytic degradation and cell-type specificity, and remained active even after disruption of cellular organization. It differed from the membrane-anchored neuregulin-1 isoforms that are responsible for axon contact-induced SC proliferation and exerted its action independently of mitogenic signaling emanating from receptor tyrosine kinases and mitogen-activated protein kinases such as ERK and JNK. Interestingly, dedifferentiation occurred without concomitant changes in the expression of Krox-20, a transcriptional enhancer of myelination, and c-Jun, an inhibitor of myelination. In sum, our data indicated the existence of cell surface axon-derived signals that override pro-differentiating cues, drive dedifferentiation and allow SCs to proliferate in response to axonal mitogens. This axonal signal may negatively regulate myelination at the onset or reversal of the differentiated state. GLIA 2017;65:851–863.

Original languageEnglish
Pages (from-to)864-882
Number of pages19
Issue number6
StatePublished - Jun 2017

Bibliographical note

Publisher Copyright:
© 2017 Wiley Periodicals, Inc.


  • cAMP
  • cell signaling
  • cell–cell interactions
  • differentiation
  • dorsal root ganglion neurons
  • in vitro systems
  • myelination
  • proliferation

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience


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