Bacterial lipopolysaccharide induced B cell activation is mediated via a phosphatidylinositol 3-kinase dependent signaling pathway

Chandrasekar Venkataraman, Gopi Shankar, Goutam Sen, Subbarao Bondada

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Bacterial lipopolysaccharide (LPS) is a potent stimulant of B cells and macrophages. LPS induces B cell proliferation and differentiation into antibody secreting cells. In addition, LPS also stimulates IL-6 secretion in mature B cells and in immature B cell lines such as WEHI-231. Although sufficient literature is available on LPS induced signaling events in monocytes and macrophages, the mechanisms involved in LPS induced B cell activation are not well understood. In this report, it is shown that both LPS mediated B cell proliferation and IL-6 secretion are dependent on phosphatidylinositol 3-kinase (PI 3-kinase) signaling pathways. The B cell specific co-receptor, CD19 is not tyrosine phosphorylated in LPS stimulated B cells. Thus, in contrast to B cell antigen receptor (BCR) signaling, the activation of PI 3-kinase appears not to be related to the recruitment of PI 3-kinase to tyrosine phosphorylated CD19. This is the first demonstration of the importance of PI 3-kinase signaling pathway in LPS mediated B lymphocyte activation.

Original languageEnglish
Pages (from-to)233-238
Number of pages6
JournalImmunology Letters
Volume69
Issue number2
DOIs
StatePublished - Aug 3 1999

Bibliographical note

Funding Information:
We thank Dr Ralph Chelvarajan for critical review of the manuscript and Mr Darrell Robertson for his technical assistance. This work was supported by Grants AI 21490 and AI 05731 to S.B. from the National Institutes of Health.

Keywords

  • B lymphocytes
  • Lipopolysaccharide
  • PI 3-kinase

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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