Bacterial Monitoring in Vials Using a Spectrophotometric Assimilation Method

Leonard J. Galante, Michael A. Brinkley, Robert A. Lodder

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Aseptic-filling processes are often used with fragile parenteral products that might be destroyed by terminal autoclaving. However, aseptic filling is not as effective as autoclaving in reducing contamination. As a result, time-consuming microbiological methods and turbidimetry are employed currently as product inspection techniques, but these processes can destroy the product and might not detect low levels of contamination. Thus, near-infrared (IR) light scattering was evaluated in this study as a new method for determining low levels of contamination noninvasively and nondestructively. A new parallel mathematical technique was used in conjunction with near-IR spectrophotometry to detect successfully contamination by several species of bacteria through intact glass vials. Using the near-IR method, products can be evaluated without introducing contamination, preserving the sample vial for dispensing or evaluation by another method.

Original languageEnglish
Pages (from-to)357-364
Number of pages8
JournalPharmaceutical Research
Issue number3
StatePublished - Mar 1992


  • bacteria
  • contamination
  • near-infrared
  • parenteral products
  • scattering
  • sterility

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry
  • Pharmacology (medical)


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