Basal brain oxidative and nitrative stress levels are finely regulated by the interplay between superoxide dismutase 2 and p53

Eugenio Barone, Giovanna Cenini, Fabio Di Domenico, Teresa Noel, Chi Wang, Marzia Perluigi, Daret K. St Clair, D. Allan Butterfield

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Superoxide dismutases (SODs) are the primary reactive oxygen species (ROS)-scavenging enzymes of the cell and catalyze the dismutation of superoxide radicals O2- to H2O2 and molecular oxygen (O2). Among the three forms of SOD identified, manganese-containing SOD (MnSOD, SOD2) is a homotetramer located wholly in the mitochondrial matrix. Because of the SOD2 strategic location, it represents the first mechanism of defense against the augmentation of ROS/reactive nitrogen species levels in the mitochondria for preventing further damage. This study seeks to understand the effects that the partial lack (SOD2-/+) or the overexpression (TgSOD2) of MnSOD produces on oxidative/nitrative stress basal levels in different brain isolated cellular fractions (i.e., mitochondrial, nuclear, cytosolic) as well as in the whole-brain homogenate. Furthermore, because of the known interaction between SOD2 and p53 protein, this study seeks to clarify the impact that the double mutation has on oxidative/nitrative stress levels in the brain of mice carrying the double mutation (p53-/- × SOD2-/+ and p53-/- × TgSOD2). We show that each mutation affects mitochondrial, nuclear, and cytosolic oxidative/nitrative stress basal levels differently, but, overall, no change or reduction of oxidative/nitrative stress levels was found in the whole-brain homogenate. The analysis of well-known antioxidant systems such as thioredoxin-1 and Nrf2/HO-1/BVR-A suggests their potential role in the maintenance of the cellular redox homeostasis in the presence of changes of SOD2 and/or p53 protein levels.

Original languageEnglish
Pages (from-to)1728-1739
Number of pages12
JournalJournal of Neuroscience Research
Issue number11
StatePublished - Nov 1 2015

Bibliographical note

Publisher Copyright:
© 2015 Wiley Periodicals, Inc.


  • Biliverdin reductase-A
  • Heme oxygenase-1
  • MnSOD; p53
  • Oxidative stress
  • RRID:AB_10618757
  • RRID:AB_10850321
  • RRID:AB_1840351
  • RRID:AB_2049199
  • RRID:AB_2256876
  • RRID:AB_476744
  • RRID:AB_881705
  • RRID:AB_958795

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience


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