Bi-phasic CD34+ cell mobilization of a syngeneic donor during prolonged G-CSF delivery

S. M. Arnold, G. Van Zant, G. Phillips

Research output: Contribution to journalArticlepeer-review


Background: G-CSF administration over 10 days and neutrophil cytapheresis have been reported in the literature, but the kinetics of CD34+ cells in this situation is unclear. Case: A 42-year-old female underwent syngeneic transplantation for metastatic breast cancer. The recipient was in critical condition peri-transplant, therefore the donor received G-CSF for 13 days, during which eight cytaphereses for both PBPC and neutrophils were performed. Two peaks in CD34+ cells were noted; the first on Day 5 and the second on Days 10-13 of G-CSF administration; a total of 11.6 × 106/kg CD34+ cells and 38.11 × 108/kg neutrophils were infused. The recipient's ANC exceeded 0.1 × 109/L on Day +3. Discussion: To our knowledge, this is the longest reported cytapheresis of CD34+ cells from a normal donor. The bi-phasic pattern in the cytapheresis product is also of interest. It is an unusual pattern that suggests a profound and complicated alteration in the marrow progenitor cell pool. If substantiated, this finding may offer an alternative cytapheresis schedule for donors.

Original languageEnglish
Pages (from-to)119-122
Number of pages4
Issue number2
StatePublished - 1999


  • Cytapheresis
  • Progenitors
  • Syngeneic
  • Transplantation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Genetics(clinical)
  • Cell Biology
  • Transplantation
  • Cancer Research


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