Binding and two-dimensional crystallization of streptavidin at the air/water interface via engineered Cu-IDA chelator lipids

Kevin M. Maloney; William R. Schief; Daniel W. Pack; Wolfgang Frey; Frances H. Arnold; Viola Vogel

doi:10.1016/S0010-8545(98)00174-X

Binding and two-dimensional crystallization of streptavidin at the air/water interface via engineered Cu-IDA chelator lipids

Kevin M. Maloney, William R. Schief, Daniel W. Pack, Wolfgang Frey, Frances H. Arnold, Viola Vogel

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Metal-chelating iminodiacetate (IDA) lipid monolayers at the air/water interface have been engineered to promote the 2D crystallization of proteins which display solvent-accessible histidines. The physical properties of IDA lipid monolayers have been specifically tailored to allow the binding and 2D assembly of added proteins. Fluorescently labeled IDA lipid monolayers allow us to monitor the formation of the protein crystals optically. Here we review our efforts in the interfacial crystallization of streptavidin as monitored using fluorescence, Brewster angle and transmission electron microscopy and discuss future challenges in this area.

Original language	English
Pages (from-to)	3-18
Number of pages	16
Journal	Coordination Chemistry Reviews
Volume	183
Issue number	1
DOIs	https://doi.org/10.1016/S0010-8545(98)00174-X
State	Published - Mar 1 1999

Bibliographical note

Funding Information:
We gratefully acknowledge support from the Office of Naval Research (FHA), the Army Research Office (FHA), NASA (NAG8-1149 to VV), a National Institute of General Medical Sciences, National Institutes of Health post-doctoral fellowship (1 F32 GM 1632, KMM), and an NIH Training Grant in Biotechnology and Molecular Biophysics (WRS).

Funding

We gratefully acknowledge support from the Office of Naval Research (FHA), the Army Research Office (FHA), NASA (NAG8-1149 to VV), a National Institute of General Medical Sciences, National Institutes of Health post-doctoral fellowship (1 F32 GM 1632, KMM), and an NIH Training Grant in Biotechnology and Molecular Biophysics (WRS).

Funders	Funder number
National Institutes of Health (NIH)	1 F32 GM 1632
Office of Naval Research
National Institute of General Medical Sciences
National Aeronautics and Space Administration	NAG8-1149
Army Research Office
AGS Foundation for Health in Aging

Keywords

Air/water interface
Brewster angle microscopy
Cu(II)
Fluorescence microscopy
Iminodiacetate
Metal-chelating lipids
Monolayers

ASJC Scopus subject areas

General Chemistry
Materials Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry

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10.1016/S0010-8545(98)00174-X

Cite this

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title = "Binding and two-dimensional crystallization of streptavidin at the air/water interface via engineered Cu-IDA chelator lipids",

abstract = "Metal-chelating iminodiacetate (IDA) lipid monolayers at the air/water interface have been engineered to promote the 2D crystallization of proteins which display solvent-accessible histidines. The physical properties of IDA lipid monolayers have been specifically tailored to allow the binding and 2D assembly of added proteins. Fluorescently labeled IDA lipid monolayers allow us to monitor the formation of the protein crystals optically. Here we review our efforts in the interfacial crystallization of streptavidin as monitored using fluorescence, Brewster angle and transmission electron microscopy and discuss future challenges in this area.",

keywords = "Air/water interface, Brewster angle microscopy, Cu(II), Fluorescence microscopy, Iminodiacetate, Metal-chelating lipids, Monolayers",

author = "Maloney, {Kevin M.} and Schief, {William R.} and Pack, {Daniel W.} and Wolfgang Frey and Arnold, {Frances H.} and Viola Vogel",

note = "Funding Information: We gratefully acknowledge support from the Office of Naval Research (FHA), the Army Research Office (FHA), NASA (NAG8-1149 to VV), a National Institute of General Medical Sciences, National Institutes of Health post-doctoral fellowship (1 F32 GM 1632, KMM), and an NIH Training Grant in Biotechnology and Molecular Biophysics (WRS).",

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AU - Maloney, Kevin M.

AU - Schief, William R.

AU - Pack, Daniel W.

AU - Frey, Wolfgang

AU - Arnold, Frances H.

AU - Vogel, Viola

N1 - Funding Information: We gratefully acknowledge support from the Office of Naval Research (FHA), the Army Research Office (FHA), NASA (NAG8-1149 to VV), a National Institute of General Medical Sciences, National Institutes of Health post-doctoral fellowship (1 F32 GM 1632, KMM), and an NIH Training Grant in Biotechnology and Molecular Biophysics (WRS).

PY - 1999/3/1

Y1 - 1999/3/1

N2 - Metal-chelating iminodiacetate (IDA) lipid monolayers at the air/water interface have been engineered to promote the 2D crystallization of proteins which display solvent-accessible histidines. The physical properties of IDA lipid monolayers have been specifically tailored to allow the binding and 2D assembly of added proteins. Fluorescently labeled IDA lipid monolayers allow us to monitor the formation of the protein crystals optically. Here we review our efforts in the interfacial crystallization of streptavidin as monitored using fluorescence, Brewster angle and transmission electron microscopy and discuss future challenges in this area.

AB - Metal-chelating iminodiacetate (IDA) lipid monolayers at the air/water interface have been engineered to promote the 2D crystallization of proteins which display solvent-accessible histidines. The physical properties of IDA lipid monolayers have been specifically tailored to allow the binding and 2D assembly of added proteins. Fluorescently labeled IDA lipid monolayers allow us to monitor the formation of the protein crystals optically. Here we review our efforts in the interfacial crystallization of streptavidin as monitored using fluorescence, Brewster angle and transmission electron microscopy and discuss future challenges in this area.

KW - Air/water interface

KW - Brewster angle microscopy

KW - Cu(II)

KW - Fluorescence microscopy

KW - Iminodiacetate

KW - Metal-chelating lipids

KW - Monolayers

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Binding and two-dimensional crystallization of streptavidin at the air/water interface via engineered Cu-IDA chelator lipids

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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