Binding of serum amyloid P-component (SAP) by amyloid fibrils

M. B. Pepys, R. F. Dyck, F. C. de Beer, M. Skinner, A. S. Cohen

Research output: Contribution to journalArticlepeer-review

224 Scopus citations


An assay for circulating immune complexes is described which uses radiolabelled bovine conglutinin as ligand and polyethylene glycol precipitation as the technique for separating bound ligands. The technique is simple to perform and gives good sensitivity detecting artificial immune complexes. Its use in detecting complexes in systemic lupus erythematosus and Burkitt's lymphoma is described and it is compared with the Clq binding assay also performed with polyethylene glycol. It is suggested that the simultaneous performance of polyethylene glycol assays using radiolabelled Clq and radiolabelled conglutinin may be an advantageous method for screening sera for the presence of immune complexes.

Original languageEnglish
Pages (from-to)284-293
Number of pages10
JournalClinical and Experimental Immunology
Issue number2
StatePublished - 1979

ASJC Scopus subject areas

  • Medicine (all)


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