Bioactive metabolites from the ruminal bacterium Enterobacter amnigenus ZIH

Imene Zendah El Euch, Khaled A. Shaaban, Naheed Riaz, Muhammad Bahi, Mohamed Shaaban

Research output: Contribution to journalArticlepeer-review


Bioassay-guided fractionation and chromatographic purification of the crude extract of ruminal bacterium Enterobacter amnigenus ZIH (isolated from cattle) produce one new microbial product:butyl-α-D-glucopyranoside together with eleven known compounds including (S)-brevinic acid, 3-(hydroxyacetyl)-indole, Nß-acetyltryptamine, tyrosol, phenol, tryptophol, indole-3-lactic acid, uracil, adenine and two diketopiperazines cyclo-(Phe, Pro) and cyclo-(Leu, Pro). The complete NMR assignments of butyl-α-D-glucopyranoside was done-using different spectroscopic (1H,13C,1H-1H COSY, HMQC, and HMBC) and spectrometric methods (ESI-MS, HRESI-MS). Compounds 1a and 2 reported for the first time from a natural source. The bacterial extract exhibited high cytotoxicity against brine shrimp and moderate antimicrobial activity against a diverse set of pathogenic bacteria strains.

Original languageEnglish
Pages (from-to)222-228
Number of pages7
JournalIndian Journal of Natural Products and Resources
Issue number3
StatePublished - Sep 2018

Bibliographical note

Publisher Copyright:
© 2019, National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved.


  • Biological activities
  • Brevinic acid
  • Butyl-glucoside
  • Enterobacter amnigenus ZIH
  • Ruminal bacteria

ASJC Scopus subject areas

  • Food Science
  • Agronomy and Crop Science
  • Plant Science


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