Bioactive secondary metabolites from a new terrestrial streptomyces sp. TN262

Lobna Elleuch, Mohamed Shaaban, Slim Smaoui, Lotfi Mellouli, Ines Karray-Rebai, Lilia Fourati-Ben Fguira, Khaled A. Shaaban, Hartmut Laatsch

Research output: Contribution to journalArticlepeer-review

80 Scopus citations


During our search for Streptomyces spp. as new producers of bioactive secondary metabolites, the ethyl acetate extract of the new terrestrial Streptomyces isolate TN262 delivered eight antimicrobially active compounds. They were identified as 1-acetyl-β-carboline (1), tryptophol (2), cineromycin B (3), 2,3-dihydrocineromycin B (4), cyclo-(tyrosylprolyl) (5), 3-(hydroxyacetyl)-indole (6), brevianamide F (7), and cis-cyclo-(l-prolyl-l- leucyl) (8). Three further metabolites were detected in the unpolar fractions using GC-MS and tentatively assigned as benzophenone (9), N-butyl- benzenesulfonamide (10), and hexanedioic acid-bis-(2-ethylhexyl) ester (11). This last compound is known as plasticizer derivatives, but it has never been described from natural sources. In this article, we describe the identification of the new Streptomyces sp. isolate TN262 using its cultural characteristics, the nucleotide sequence of the corresponding 16S rRNA gene and the phylogenetic analysis, followed by optimization, large-scale fermentation, isolation of the bioactive constituents, and determination of their structures. The biological activity of compounds (2), (3), (4), and those of the unpolar fractions was addressed as well.

Original languageEnglish
Pages (from-to)579-593
Number of pages15
JournalApplied Biochemistry and Biotechnology
Issue number2
StatePublished - Sep 2010


  • Antimicrobial activities
  • Identification
  • Macrolides
  • Natural hexanedioic acid-bis-(2-ethylhexyl) ester
  • New Streptomyces sp.TN262

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology


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