Background: Plant-derived endophytic actinobacteria are the center of attention due to their capacity to produce diverse antimicrobial and anticancer compounds and their metabolites influence plant growth. Methods: In this study, 40 endophytic actinobacteria strains were isolated from the roots of eight medicinal plants used as folk medicine in South Asian region. The isolates were characterized morphologically, biochemically and physiologically and the genus level identification of the selected strains was done by 16SrRNA gene sequencing. In small scale cultivation (50ml broth), the isolates were grown in A-medium to prepare the crude extracts. These crude extracts were subsequently evaluated for their antimicrobial, anticancer and antioxidant activity and the metabolomics profile of each of the extract was determined by TLC and HPLC-UV/MS. Results: The taxonomic studies showed that the isolates belong to the group actinobacteria based on their morphological and physiological characteristics and the 16SrRNA gene sequencing of the selected strains identified the genera including Streptomyces, Micromonospora and Nocardia. Cumulatively,53% of extracts exhibited anti-Gram-(+) activity,47% exhibited anti-Gram-(-) activity,32% exhibited antifungal activity and 30% were cytotoxic to PC3 and A549 cancer cell lines and most of the extracts have shown antioxidant activity greater than 50%. The metabolomics analysis predicted the presence of an array of low molecular weight metabolites and indicated the promising isolates in collection for further studies for novel bioactive metabolite isolation and structure elucidation. Conclusion: Overall the study provides an overview of the endophytic actinobacteria residing in the roots of the selected medicinal plants prevalent in south Asian region and their potential to produce the medicinally and biotechnologically useful compounds.
|Number of pages||10|
|Journal||Advancements in Life Sciences|
|State||Published - Jun 2023|
Bibliographical noteFunding Information:
This work was supported by Higher Education Commission (HEC) Pakistan grant (HEC-NRPU-Project 2121). The work was also supported by NIH grant R37 AI52218 (JST), the UK Center of Biomedical Research Excellence (COBRE) in Translational Chemical Biology (P20 GM130456) and the University of Kentucky College of Pharmacy and Center for Clinical and Translational Sciences (UL1 TR000117 and UL1 TR001998).The data presented in this manuscript is part of the PhD thesis of author Ashba Hassan.
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- Anticancer Compounds
- Antimicrobial Compounds
- Endophytic Actinobacteria
- Metabolomic Profiling
- Natural Products
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology (all)
- Veterinary (all)