Biochemical and physiological flexibility accompanies reduced cellulose biosynthesis in Brachypodium cesa1S830N

Chad Brabham, Abhishek Singh, Jozsef Stork, Ying Rong, Indrajit Kumar, Kazuhiro Kikuchi, Yaroslava G. Yingling, Thomas P. Brutnell, Jocelyn K.C. Rose, Seth Debolt

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Here, we present a study into the mechanisms of primary cell wall cellulose formation in grasses, using the model cereal grass Brachypodium distachyon. The exon found adjacent to the BdCESA1 glycosyltransferase QXXRW motif was targeted using Targeting Induced Local Lesions in Genomes (TILLING) and sequencing candidate amplicons in multiple parallel reactions (SCAMPRing) leading to the identification of the Bdcesa1S830N allele. Plants carrying this missense mutation exhibited a significant reduction in crystalline cellulose content in tissues that rely on the primary cell wall for biomechanical support. However, Bdcesa1S830N plants failed to exhibit the predicted reduction in plant height. In a mechanism unavailable to eudicotyledons, B. distachyon plants homozygous for the Bdcesa1S830N allele appear to overcome the loss of internode expansion anatomically by increasing the number of nodes along the stem. Stem biomechanics were resultantly compromised in Bdcesa1S830N. The Bdcesa1S830N missense mutation did not interfere with BdCESA1 gene expression. However, molecular dynamic simulations of the CELLULOSE SYNTHASE A (CESA) structure with modelled membrane interactions illustrated that Bdcesa1S830N exhibited structural changes in the translated gene product responsible for reduced cellulose biosynthesis. Molecular dynamic simulations showed that substituting S830N resulted in a stabilizing shift in the flexibility of the class specific region arm of the core catalytic domain of CESA, revealing the importance of this motion to protein function.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalAoB PLANTS
Volume11
Issue number5
DOIs
StatePublished - Oct 1 2019

Bibliographical note

Funding Information:
Computational work was supported by The Center for Lignocellulose Structure and Formation, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Award Number DE-SC0001090.

Funding Information:
This research was supported by the United States National Science Foundation (NSF) 1826715 (SD), USDA Hatch Funding (SD) and Department of Energy DOE-FOA 10-0000368 (SD, JKCR, TB).

Publisher Copyright:
© 2019 The Author(s) 2019. Published by Oxford University Press on behalf of the Annals of Botany Company.

Keywords

  • Biomechanics
  • CESA
  • cellular expansion
  • cellulose
  • class-specific region
  • molecular dynamics

ASJC Scopus subject areas

  • Plant Science

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