Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction

Shuting Zhao; Zhaobin Xu; Hai Wang; Benjamin E. Reese; Liubov V. Gushchina; Meng Jiang; Pranay Agarwal; Jiangsheng Xu; Mingjun Zhang; Rulong Shen; Zhenguo Liu; Noah Weisleder; Xiaoming He

doi:10.1038/ncomms13306

Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction

Shuting Zhao, Zhaobin Xu, Hai Wang, Benjamin E. Reese, Liubov V. Gushchina, Meng Jiang, Pranay Agarwal, Jiangsheng Xu, Mingjun Zhang, Rulong Shen, Zhenguo Liu, Noah Weisleder, Xiaoming He

Research output: Contribution to journal › Article › peer-review

98 Scopus citations

Abstract

It is difficult to achieve minimally invasive injectable cell delivery while maintaining high cell retention and animal survival for in vivo stem cell therapy of myocardial infarction. Here we show that pluripotent stem cell aggregates pre-differentiated into the early cardiac lineage and encapsulated in a biocompatible and biodegradable micromatrix, are suitable for injectable delivery. This method significantly improves the survival of the injected cells by more than six-fold compared with the conventional practice of injecting single cells, and effectively prevents teratoma formation. Moreover, this method significantly enhances cardiac function and survival of animals after myocardial infarction, as a result of a localized immunosuppression effect of the micromatrix and the in situ cardiac regeneration by the injected cells.

Original language	English
Article number	13306
Journal	Nature Communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms13306
State	Published - Oct 27 2016

Bibliographical note

Publisher Copyright:
© The Author(s) 2016.

Funding

Funders	Funder number
National Heart, Lung, and Blood Institute Family Blood Pressure Program	R01HL094650
National Heart, Lung, and Blood Institute Family Blood Pressure Program

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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title = "Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction",

abstract = "It is difficult to achieve minimally invasive injectable cell delivery while maintaining high cell retention and animal survival for in vivo stem cell therapy of myocardial infarction. Here we show that pluripotent stem cell aggregates pre-differentiated into the early cardiac lineage and encapsulated in a biocompatible and biodegradable micromatrix, are suitable for injectable delivery. This method significantly improves the survival of the injected cells by more than six-fold compared with the conventional practice of injecting single cells, and effectively prevents teratoma formation. Moreover, this method significantly enhances cardiac function and survival of animals after myocardial infarction, as a result of a localized immunosuppression effect of the micromatrix and the in situ cardiac regeneration by the injected cells.",

author = "Shuting Zhao and Zhaobin Xu and Hai Wang and Reese, \{Benjamin E.\} and Gushchina, \{Liubov V.\} and Meng Jiang and Pranay Agarwal and Jiangsheng Xu and Mingjun Zhang and Rulong Shen and Zhenguo Liu and Noah Weisleder and Xiaoming He",

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AU - Zhao, Shuting

AU - Xu, Zhaobin

AU - Wang, Hai

AU - Reese, Benjamin E.

AU - Gushchina, Liubov V.

AU - Jiang, Meng

AU - Agarwal, Pranay

AU - Xu, Jiangsheng

AU - Zhang, Mingjun

AU - Shen, Rulong

AU - Liu, Zhenguo

AU - Weisleder, Noah

AU - He, Xiaoming

PY - 2016/10/27

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N2 - It is difficult to achieve minimally invasive injectable cell delivery while maintaining high cell retention and animal survival for in vivo stem cell therapy of myocardial infarction. Here we show that pluripotent stem cell aggregates pre-differentiated into the early cardiac lineage and encapsulated in a biocompatible and biodegradable micromatrix, are suitable for injectable delivery. This method significantly improves the survival of the injected cells by more than six-fold compared with the conventional practice of injecting single cells, and effectively prevents teratoma formation. Moreover, this method significantly enhances cardiac function and survival of animals after myocardial infarction, as a result of a localized immunosuppression effect of the micromatrix and the in situ cardiac regeneration by the injected cells.

AB - It is difficult to achieve minimally invasive injectable cell delivery while maintaining high cell retention and animal survival for in vivo stem cell therapy of myocardial infarction. Here we show that pluripotent stem cell aggregates pre-differentiated into the early cardiac lineage and encapsulated in a biocompatible and biodegradable micromatrix, are suitable for injectable delivery. This method significantly improves the survival of the injected cells by more than six-fold compared with the conventional practice of injecting single cells, and effectively prevents teratoma formation. Moreover, this method significantly enhances cardiac function and survival of animals after myocardial infarction, as a result of a localized immunosuppression effect of the micromatrix and the in situ cardiac regeneration by the injected cells.

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Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction

Abstract

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