Biofunctional membranes: electron paramagnetic resonance studies of the active site structure of enzymes site-specifically immobilized onto polymeric supports through molecular recognition

D. Allan Butterfield; Ram Subramaniam; Dibakar Bhattacharyya; Shekhar Vishwanath; Wei Huang; Leonidas Bachas

Biofunctional membranes: electron paramagnetic resonance studies of the active site structure of enzymes site-specifically immobilized onto polymeric supports through molecular recognition

D. Allan Butterfield, Ram Subramaniam, Dibakar Bhattacharyya, Shekhar Vishwanath, Wei Huang, Leonidas Bachas

Research output: Contribution to journal › Conference article › peer-review

4 Scopus citations

Abstract

Results are presented of studies on spin-labeled enzymes immobilized onto polymeric supports using site-specific immobilization. A different way to use molecular recognition to immobilize subtilisin is to utilize molecular biology, and site-directed mutagenesis in particular. It is shown that utilizing the principles of molecular recognition, one can immobilize enzymes onto polymeric supports making the resulting biofunctional membrane more active than with random immobilization.

Original language	English
Pages (from-to)	602-603
Number of pages	2
Journal	Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering
Volume	76
State	Published - 1997
Event	Proceedings of the 1997 Spring ACS Meeting - San Francisco, CA, USA Duration: Apr 13 1997 → Apr 17 1997

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Polymers and Plastics

Cite this

Butterfield, D. A., Subramaniam, R., Bhattacharyya, D., Vishwanath, S., Huang, W., & Bachas, L. (1997). Biofunctional membranes: electron paramagnetic resonance studies of the active site structure of enzymes site-specifically immobilized onto polymeric supports through molecular recognition. Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering, 76, 602-603.

Butterfield, D. Allan ; Subramaniam, Ram ; Bhattacharyya, Dibakar et al. / Biofunctional membranes : electron paramagnetic resonance studies of the active site structure of enzymes site-specifically immobilized onto polymeric supports through molecular recognition. In: Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. 1997 ; Vol. 76. pp. 602-603.

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title = "Biofunctional membranes: electron paramagnetic resonance studies of the active site structure of enzymes site-specifically immobilized onto polymeric supports through molecular recognition",

abstract = "Results are presented of studies on spin-labeled enzymes immobilized onto polymeric supports using site-specific immobilization. A different way to use molecular recognition to immobilize subtilisin is to utilize molecular biology, and site-directed mutagenesis in particular. It is shown that utilizing the principles of molecular recognition, one can immobilize enzymes onto polymeric supports making the resulting biofunctional membrane more active than with random immobilization.",

author = "Butterfield, {D. Allan} and Ram Subramaniam and Dibakar Bhattacharyya and Shekhar Vishwanath and Wei Huang and Leonidas Bachas",

year = "1997",

language = "English",

volume = "76",

pages = "602--603",

note = "Proceedings of the 1997 Spring ACS Meeting ; Conference date: 13-04-1997 Through 17-04-1997",

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Butterfield, DA, Subramaniam, R, Bhattacharyya, D, Vishwanath, S, Huang, W & Bachas, L 1997, 'Biofunctional membranes: electron paramagnetic resonance studies of the active site structure of enzymes site-specifically immobilized onto polymeric supports through molecular recognition', Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering, vol. 76, pp. 602-603.

Biofunctional membranes: electron paramagnetic resonance studies of the active site structure of enzymes site-specifically immobilized onto polymeric supports through molecular recognition. / Butterfield, D. Allan; Subramaniam, Ram; Bhattacharyya, Dibakar et al.
In: Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering, Vol. 76, 1997, p. 602-603.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Biofunctional membranes

T2 - Proceedings of the 1997 Spring ACS Meeting

AU - Butterfield, D. Allan

AU - Subramaniam, Ram

AU - Bhattacharyya, Dibakar

AU - Vishwanath, Shekhar

AU - Huang, Wei

AU - Bachas, Leonidas

PY - 1997

Y1 - 1997

N2 - Results are presented of studies on spin-labeled enzymes immobilized onto polymeric supports using site-specific immobilization. A different way to use molecular recognition to immobilize subtilisin is to utilize molecular biology, and site-directed mutagenesis in particular. It is shown that utilizing the principles of molecular recognition, one can immobilize enzymes onto polymeric supports making the resulting biofunctional membrane more active than with random immobilization.

AB - Results are presented of studies on spin-labeled enzymes immobilized onto polymeric supports using site-specific immobilization. A different way to use molecular recognition to immobilize subtilisin is to utilize molecular biology, and site-directed mutagenesis in particular. It is shown that utilizing the principles of molecular recognition, one can immobilize enzymes onto polymeric supports making the resulting biofunctional membrane more active than with random immobilization.

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UR - http://www.scopus.com/inward/citedby.url?scp=0030709602&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:0030709602

SN - 0743-0515

VL - 76

SP - 602

EP - 603

JO - Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering

JF - Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering

Y2 - 13 April 1997 through 17 April 1997

ER -

Butterfield DA, Subramaniam R, Bhattacharyya D, Vishwanath S, Huang W, Bachas L. Biofunctional membranes: electron paramagnetic resonance studies of the active site structure of enzymes site-specifically immobilized onto polymeric supports through molecular recognition. Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. 1997;76:602-603.

Biofunctional membranes: electron paramagnetic resonance studies of the active site structure of enzymes site-specifically immobilized onto polymeric supports through molecular recognition

Abstract

ASJC Scopus subject areas

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