Biolistic transformation of sorghum using a rice chitinase gene

H. Zhu, S. Muthukrishnan, S. Krishnaveni, G. Wilde, J. M. Jeoung, G. H. Liang

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70 Scopus citations


A rice chitinase gene, G11, which may have a protective role against fungal pathogens, was introduced into a sorghum inbred 'Tx430'. Calli derived from immature zygotic embryos were bombarded with tungsten particles coated with a plasmid DNA containing this gene and the bar gene as a selectable marker. After transformation, six fertile transgenic plants were obtained from a total of 1,100 bombarded calli. Molecular analyses by phosphinothricin acetyltransferase (PAT) activity assay, Southern blotting, and western blotting confirmed the presence and expression of the selectable bar gene and the rice chitinase gene in primary transgenic plants (T0). However, gene silencing occurred in a few T0 plants at certain growth stages. Progeny analysis showed that the introduced chitinase gene was inherited and segregated among T1 plants (progeny of selfed T0 plants). Western blot analysis showed a 3:1 Mendelian segregation of the rice chitinase gene in T1 progeny, suggesting that the active chitinase gene(s) was inserted in a single locus in the T0 plants.

Original languageEnglish
Pages (from-to)243-252
Number of pages10
JournalJournal of Genetics and Breeding
Issue number3
StatePublished - Sep 1998


  • Gene silencing
  • Southern and western analyses
  • Tissue culture
  • Transgenic plants
  • Tx 430

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Horticulture


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