Biomarker panel discriminates diabetics with and without periodontitis pre- and post-therapy

Craig S. Miller, Xiuhua Ding, Radha Nagarajan, Dolph R. Dawson, Jeffrey L Ebersole

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Background and Objective: Biological regulators of periodontal inflammation, collagen degradation, and insulin resistance have not been determined in association with severity of periodontitis and response to periodontal treatment in diabetics. Our objective was to determine whether type 2 diabetes (T2DM) patients with periodontal disease present a distinct salivary biomarker profile compared with T2DM patients without periodontal disease and healthy subjects (without diabetes and periodontitis) pre- and post-nonsurgical therapy. Methods: Clinical parameters of periodontal health and whole unstimulated saliva were collected from 92 participants (31 Not Periodontitis, NP; 32 T2DM without periodontitis, DWoP; and 29 with T2DM with periodontitis, DWP) at baseline. The T2DM groups received scaling and root planning (SRP) and provided saliva at 6-week follow-up. Salivary concentrations of interleukin (IL)-1β, IL-6, matrix metalloproteinase-8 (MMP-8), and resistin were measured by immunoassay. Results: The DWP group had significantly more disease and higher salivary concentrations at baseline for IL-1β, MMP-8, and resistin (p's <.01) compared with DWoP and NP. SRP resulted in significant improvement in periodontal parameters for the T2DM groups; however, more disease persisted (p <.001), and IL-1β, MMP-8, and resistin concentrations remained significantly higher in the DWP than the DWoP group (p <.01) at 6 weeks post-treatment. Principal component analysis demonstrated the DWoP group appeared more biologically similar to the NP group than the DWP group. Concentrations of these salivary biomarkers increased with increasing periodontal disease severity (p <.05) in this study population. Conclusion: Salivary concentrations of IL-1β, MMP-8, and resistin appear to serve as biomarkers of periodontal status pre- and post-treatment, irrespective of diabetes status.

Original languageEnglish
Pages (from-to)493-502
Number of pages10
JournalJournal of Periodontal Research
Issue number3
StatePublished - Jun 2023

Bibliographical note

Publisher Copyright:
© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.


  • biological markers
  • diabetes
  • metalloproteases
  • periodontitis
  • saliva

ASJC Scopus subject areas

  • Periodontics


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