TY - JOUR
T1 - Biomodulation of Inflammatory Cytokines Related to Oral Mucositis by Low-Level Laser Therapy
AU - Basso, Fernanda G.
AU - Pansani, Taisa N.
AU - Soares, Diana G.
AU - Scheffel, Débora L.
AU - Bagnato, Vanderlei S.
AU - De Souza Costa, Carlos Alberto
AU - Hebling, Josimeri
N1 - Publisher Copyright:
© 2015 The American Society of Photobiology.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - This study evaluated the effects of LLLT on the expression of inflammatory cytokines related to the development of oral mucositis by gingival fibroblasts. Primary gingival fibroblasts were seeded on 24-well plates (105 cells/well) for 24 h. Fresh serum-free culture medium (DMEM) was then added, and cells were placed in contact with LPS (Escherichia coli, 1 μg mL-1), followed by LLLT irradiation (LaserTABLE - InGaAsP diode prototype - 780 nm, 25 mW) delivering 0, 0.5, 1.5 or 3 J cm-. Cells without contact with LPS were also irradiated with the same energy densities. Gene expression of TNF-α, IL-1β, IL-6 and IL-8 was evaluated by Real-Time PCR, and protein synthesis of these cytokines was determined by enzyme-linked immunosorbent (ELISA) assay. Data were statistically analyzed by the Kruskal-Wallis test, complemented by the Mann-Whitney test (P < 0.05). LPS treatment increased the gene expression and protein synthesis of TNF-α, IL-6 and IL-8, while the expression of IL-1β was not affected. For LPS-treated groups, LLLT promoted significant decreases in the expression of TNF-α, IL-6, and IL-8 at 1.5 J cm-2 and 3 J cm-2. These results demonstrate that LLLT promoted a beneficial biomodulatory effect on the expression of inflammatory cytokines related to oral mucositis by human gingival fibroblasts.
AB - This study evaluated the effects of LLLT on the expression of inflammatory cytokines related to the development of oral mucositis by gingival fibroblasts. Primary gingival fibroblasts were seeded on 24-well plates (105 cells/well) for 24 h. Fresh serum-free culture medium (DMEM) was then added, and cells were placed in contact with LPS (Escherichia coli, 1 μg mL-1), followed by LLLT irradiation (LaserTABLE - InGaAsP diode prototype - 780 nm, 25 mW) delivering 0, 0.5, 1.5 or 3 J cm-. Cells without contact with LPS were also irradiated with the same energy densities. Gene expression of TNF-α, IL-1β, IL-6 and IL-8 was evaluated by Real-Time PCR, and protein synthesis of these cytokines was determined by enzyme-linked immunosorbent (ELISA) assay. Data were statistically analyzed by the Kruskal-Wallis test, complemented by the Mann-Whitney test (P < 0.05). LPS treatment increased the gene expression and protein synthesis of TNF-α, IL-6 and IL-8, while the expression of IL-1β was not affected. For LPS-treated groups, LLLT promoted significant decreases in the expression of TNF-α, IL-6, and IL-8 at 1.5 J cm-2 and 3 J cm-2. These results demonstrate that LLLT promoted a beneficial biomodulatory effect on the expression of inflammatory cytokines related to oral mucositis by human gingival fibroblasts.
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U2 - 10.1111/php.12445
DO - 10.1111/php.12445
M3 - Article
C2 - 25735212
AN - SCOPUS:84934267924
SN - 0031-8655
VL - 91
SP - 952
EP - 956
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
IS - 4
ER -