Background: Bioprospecting of actinobacteria isolated from Kubuqi desert, China for antibacterial, antifungal and cytotoxic metabolites production and their structure elucidation. Results: A total of 100 actinobacteria strains were selectively isolated from Kubuqi desert, Inner Mongolia, China. The taxonomic characterization revealed Streptomyces as the predominant genus comprising 37 different species, along with the rare actinobacterial genus Lentzea. The methanolic extracts of 60.8% of strains exhibited potent antimicrobial activities against Staphylococcus aureus, Micrococcus luteus, Bacillus subtilis, Escherichia coli, Salmonella enterica, Saccharomyces cerevisiae and high to mild in vitro cytotoxicity against PC3 (prostate cancer) and A549 (lung carcinoma) cell lines. The metabolomics analysis by TLC, HPLC-UV/vis, HPLC-MS and NMR showed the presence of compounds with molecular weights ranging from 100 to 1000 Da. The scale-up fermentation of the prioritized anti-Gram-negative strain PU-KB10–4 (Streptomyces griseoviridis), yielded three pure compounds including; griseoviridin (1; 42.0 mgL− 1) with 20 fold increased production as compared to previous reports and its crystal structure as monohydrate form is herein reported for the first time, mitomycin C (2; 0.3 mgL− 1) and a new bacterial metabolite 4-hydroxycinnamide (3; 0.59 mgL− 1). Conclusions: This is the first report of the bioprospecting and exploration of actinobacteria from Kubuqi desert and the metabolite 4-hydroxycinnamide (3) is first time isolated from a bacterial source. This study demonstrated that actinobacteria from Kubuqi desert are a potential source of novel bioactive natural products. Underexplored harsh environments like the Kubuqi desert may harbor a wider diversity of actinobacteria, particularly Streptomyces, which produce unique metabolites and are an intriguing source to develop medicinally valuable natural products.
|State||Published - Dec 2023|
Bibliographical noteFunding Information:
This work was supported by Higher Education Commission (HEC) Pakistan grant [NRPU-Project 2121] and CAMS (Chinese Academy of Medical Sciences) innovation Fund for Medical Sciences [grant numbers CAMS 2017-I2M-B&R-08 and 2017-I2M-1-012]. The work was also supported by NIH grant R37 AI52218 (JST), the UK Center of Biomedical Research Excellence (COBRE) in Translational Chemical Biology (P20 GM130456) and the University of Kentucky College of Pharmacy and Center for Clinical and Translational Sciences (UL1 TR000117 and UL1 TR001998).
© 2023, The Author(s).
- Kubuqi desert
- Natural products
ASJC Scopus subject areas
- Microbiology (medical)