Blocking mitochondrial permeability transition prevents p53 mitochondrial translocation during skin tumor promotion

Jianfeng Liu; Daret K. St. Clair; Xin Gu; Yunfeng Zhao

doi:10.1016/j.febslet.2008.03.013

Blocking mitochondrial permeability transition prevents p53 mitochondrial translocation during skin tumor promotion

Jianfeng Liu, Daret K. St. Clair, Xin Gu, Yunfeng Zhao

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

The tumor suppressor p53 can translocate into mitochondria and activate apoptosis. Here we studied whether p53 mitochondrial translocation and subsequent apoptosis were affected by blocking mitochondrial permeability transition pore using cyclosporine A (CsA) and bongkrekic acid (BA) in skin epidermal JB6 cells and skin tissues. Our results demonstrated that CsA and BA blocked TPA-induced p53 translocation, leading to protection against the loss of mitochondrial membrane potential and Complex I activity, and eventually suppression of apoptosis. Thus, our results suggest that mitochondrial permeability transition is required for p53 mitochondrial translocation.

Original language	English
Pages (from-to)	1319-1324
Number of pages	6
Journal	FEBS Letters
Volume	582
Issue number	9
DOIs	https://doi.org/10.1016/j.febslet.2008.03.013
State	Published - Apr 16 2008

Bibliographical note

Funding Information:
The authors wish to thank Dr. Nancy Colburn at NCI/NIH for providing us the mouse skin epidermal JB6 cells; Kathleen Llorens at LSU Health Sciences Center-Shreveport Research Core Facility for technical support in laser confocal experiments; Dr. Steven Pruett at LSUHSC-S for technical support on VersaDoc Imagine System; Dr. Tammy Dugas, Dr. Kenneth McMartin, Dr. Heather Kleiner, Bo Jiang, and Yan Li at LSUHSC-S for their technical supports. This work was supported in part by Grant Number R03CA128077 from the National Cancer Institute.

Funding

The authors wish to thank Dr. Nancy Colburn at NCI/NIH for providing us the mouse skin epidermal JB6 cells; Kathleen Llorens at LSU Health Sciences Center-Shreveport Research Core Facility for technical support in laser confocal experiments; Dr. Steven Pruett at LSUHSC-S for technical support on VersaDoc Imagine System; Dr. Tammy Dugas, Dr. Kenneth McMartin, Dr. Heather Kleiner, Bo Jiang, and Yan Li at LSUHSC-S for their technical supports. This work was supported in part by Grant Number R03CA128077 from the National Cancer Institute.

Funders	Funder number
National Childhood Cancer Registry – National Cancer Institute	R03CA128077

Keywords

Apoptosis
Mitochondria
p53

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2008.03.013

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title = "Blocking mitochondrial permeability transition prevents p53 mitochondrial translocation during skin tumor promotion",

abstract = "The tumor suppressor p53 can translocate into mitochondria and activate apoptosis. Here we studied whether p53 mitochondrial translocation and subsequent apoptosis were affected by blocking mitochondrial permeability transition pore using cyclosporine A (CsA) and bongkrekic acid (BA) in skin epidermal JB6 cells and skin tissues. Our results demonstrated that CsA and BA blocked TPA-induced p53 translocation, leading to protection against the loss of mitochondrial membrane potential and Complex I activity, and eventually suppression of apoptosis. Thus, our results suggest that mitochondrial permeability transition is required for p53 mitochondrial translocation.",

keywords = "Apoptosis, Mitochondria, p53",

author = "Jianfeng Liu and {St. Clair}, {Daret K.} and Xin Gu and Yunfeng Zhao",

note = "Funding Information: The authors wish to thank Dr. Nancy Colburn at NCI/NIH for providing us the mouse skin epidermal JB6 cells; Kathleen Llorens at LSU Health Sciences Center-Shreveport Research Core Facility for technical support in laser confocal experiments; Dr. Steven Pruett at LSUHSC-S for technical support on VersaDoc Imagine System; Dr. Tammy Dugas, Dr. Kenneth McMartin, Dr. Heather Kleiner, Bo Jiang, and Yan Li at LSUHSC-S for their technical supports. This work was supported in part by Grant Number R03CA128077 from the National Cancer Institute.",

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doi = "10.1016/j.febslet.2008.03.013",

language = "English",

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AU - Liu, Jianfeng

AU - St. Clair, Daret K.

AU - Gu, Xin

AU - Zhao, Yunfeng

N1 - Funding Information: The authors wish to thank Dr. Nancy Colburn at NCI/NIH for providing us the mouse skin epidermal JB6 cells; Kathleen Llorens at LSU Health Sciences Center-Shreveport Research Core Facility for technical support in laser confocal experiments; Dr. Steven Pruett at LSUHSC-S for technical support on VersaDoc Imagine System; Dr. Tammy Dugas, Dr. Kenneth McMartin, Dr. Heather Kleiner, Bo Jiang, and Yan Li at LSUHSC-S for their technical supports. This work was supported in part by Grant Number R03CA128077 from the National Cancer Institute.

PY - 2008/4/16

Y1 - 2008/4/16

N2 - The tumor suppressor p53 can translocate into mitochondria and activate apoptosis. Here we studied whether p53 mitochondrial translocation and subsequent apoptosis were affected by blocking mitochondrial permeability transition pore using cyclosporine A (CsA) and bongkrekic acid (BA) in skin epidermal JB6 cells and skin tissues. Our results demonstrated that CsA and BA blocked TPA-induced p53 translocation, leading to protection against the loss of mitochondrial membrane potential and Complex I activity, and eventually suppression of apoptosis. Thus, our results suggest that mitochondrial permeability transition is required for p53 mitochondrial translocation.

AB - The tumor suppressor p53 can translocate into mitochondria and activate apoptosis. Here we studied whether p53 mitochondrial translocation and subsequent apoptosis were affected by blocking mitochondrial permeability transition pore using cyclosporine A (CsA) and bongkrekic acid (BA) in skin epidermal JB6 cells and skin tissues. Our results demonstrated that CsA and BA blocked TPA-induced p53 translocation, leading to protection against the loss of mitochondrial membrane potential and Complex I activity, and eventually suppression of apoptosis. Thus, our results suggest that mitochondrial permeability transition is required for p53 mitochondrial translocation.

KW - Apoptosis

KW - Mitochondria

KW - p53

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JO - FEBS Letters

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Blocking mitochondrial permeability transition prevents p53 mitochondrial translocation during skin tumor promotion

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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