Bombesin induces angiogenesis and neuroblastoma growth

Jung Hee Kang, Titilope A. Ishola, Naira Baregamian, Joshua M. Mourot, Piotr G. Rychahou, B. Mark Evers, Dai H. Chung

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


Gastrin-releasing peptide (GRP), the mammalian equivalent of bombesin (BBS), is a trophic factor for highly vascular neuroblastomas; its mechanisms of action in vivo are unknown. We sought to determine the effects of BBS on the growth of neuroblastoma xenografts and on angiogenesis. BBS significantly increased the growth of SK-N-SH and BE(2)-C human neuroblastomas; tumors demonstrated increased expression of angiogenic markers, PECAM-1 and VEGF, as well as phosphorylated (p)-Akt levels. RC-3095, a BBS/GRP antagonist, attenuated BBS-stimulated tumor growth and angiogenesis in vivo. GRP or GRPR silencing significantly inhibited VEGF as well as p-Akt and p-mTOR expression in vitro. Our findings demonstrate that BBS stimulates neuroblastoma growth and the expression of angiogenic markers. Importantly, these findings suggest that novel therapeutic agents, targeting BBS-mediated angiogenesis, may be useful adjuncts in patients with advanced-stage neuroblastomas.

Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalCancer Letters
Issue number2
StatePublished - Aug 18 2007

Bibliographical note

Funding Information:
The authors thank Karen Martin for manuscript preparation and Tatsuo Uchida for statistical analysis. This work was supported by Grants RO1 DK61470, RO1 DK48498, RO1 CA104748 and PO1 DK35608 from the National Institutes of Health and an Institutional Research Grant (IRG-110376) from the American Cancer Society.


  • Angiogenesis
  • Bombesin
  • GRP
  • Neuroblastoma
  • VEGF

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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