Bone resorption and mRNA expression of IL-6 and IL-6 receptor in patients with renal osteodystrophy

M. Chris Langub; Nicholas J. Koszewski; Heather V. Turner; Marie Claude Monier-Faugere; Zhaopo Geng; Hartmut H. Malluche

doi:10.1038/ki.1996.343

Bone resorption and mRNA expression of IL-6 and IL-6 receptor in patients with renal osteodystrophy

M. Chris Langub, Nicholas J. Koszewski, Heather V. Turner, Marie Claude Monier-Faugere, Zhaopo Geng, Hartmut H. Malluche

Internal Medicine

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

The cytokine interleukin (IL-4) is a major cell regulation factor that may play an important role in the bone remodeling of patients renal failure. IL-6 exerts in action by binding to its receptor (IL-6R), which leads to transduction of a second messenger cascade within cells. In vitro as well as in vivo data point to IL-6 as an autocrine paracrine factor in bone osteoclasts. Recently, bone cells from patients with Paget's disease were found to express IL-6 and IL-6R mRNA transcripts. However, in patients with renal bone disease, there is currently no in vivo evidence that osteoclasts have the capability to express mRNA for IL-6 and IL-6R. To investigate the potential expression of IL-6 and IL-6R in bone and its relationship to bone cell activity, that crest bone biopsies were performed in patients on chronic maintenance dialysis. Messenger RNA expression of IL-4 and IL-6R was studied using in situ hybridization histochemistry, and parameters of bone turnover were determined by bone histomorphometry. In the samples studied, mRNA expression of IL-6 and IL-6R was found in osteoclasts and bone marrow cells. Furthermore, we report the novel finding of increased IL-6R mRNA expression in osteoclasts engaged in increased bone resorption. The results of the present study suggest that the cytokine IL-4 is intricately involved in osteoclastic bone resorption and that expression of its receptor. IL-65, in osteoclasts may parallel osteoclasts bone resorbing activity.

Original language	English
Pages (from-to)	515-520
Number of pages	6
Journal	Kidney International
Volume	50
Issue number	2
DOIs	https://doi.org/10.1038/ki.1996.343
State	Published - 1996

Bibliographical note

Funding Information:
Acknowledgments Funding for this project was supported in part by a grant from Dialysis Clinic Inc., Lexington, Kentucky (L-105,L-106). We are grateful to Dr. James P. Herman for allowing us to use the image analysis system and to Holli Gravatte, Kenloch F. Westberry III, and Richard M. Wheaton for valuable technical assistance. We thank Louise Tipton for her assistance in manuscript preparation.

ASJC Scopus subject areas

Nephrology

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10.1038/ki.1996.343

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@article{cf73d402db544ae293c47bd3e0e13296,

title = "Bone resorption and mRNA expression of IL-6 and IL-6 receptor in patients with renal osteodystrophy",

abstract = "The cytokine interleukin (IL-4) is a major cell regulation factor that may play an important role in the bone remodeling of patients renal failure. IL-6 exerts in action by binding to its receptor (IL-6R), which leads to transduction of a second messenger cascade within cells. In vitro as well as in vivo data point to IL-6 as an autocrine paracrine factor in bone osteoclasts. Recently, bone cells from patients with Paget's disease were found to express IL-6 and IL-6R mRNA transcripts. However, in patients with renal bone disease, there is currently no in vivo evidence that osteoclasts have the capability to express mRNA for IL-6 and IL-6R. To investigate the potential expression of IL-6 and IL-6R in bone and its relationship to bone cell activity, that crest bone biopsies were performed in patients on chronic maintenance dialysis. Messenger RNA expression of IL-4 and IL-6R was studied using in situ hybridization histochemistry, and parameters of bone turnover were determined by bone histomorphometry. In the samples studied, mRNA expression of IL-6 and IL-6R was found in osteoclasts and bone marrow cells. Furthermore, we report the novel finding of increased IL-6R mRNA expression in osteoclasts engaged in increased bone resorption. The results of the present study suggest that the cytokine IL-4 is intricately involved in osteoclastic bone resorption and that expression of its receptor. IL-65, in osteoclasts may parallel osteoclasts bone resorbing activity.",

author = "Langub, {M. Chris} and Koszewski, {Nicholas J.} and Turner, {Heather V.} and Monier-Faugere, {Marie Claude} and Zhaopo Geng and Malluche, {Hartmut H.}",

note = "Funding Information: Acknowledgments Funding for this project was supported in part by a grant from Dialysis Clinic Inc., Lexington, Kentucky (L-105,L-106). We are grateful to Dr. James P. Herman for allowing us to use the image analysis system and to Holli Gravatte, Kenloch F. Westberry III, and Richard M. Wheaton for valuable technical assistance. We thank Louise Tipton for her assistance in manuscript preparation.",

year = "1996",

doi = "10.1038/ki.1996.343",

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T1 - Bone resorption and mRNA expression of IL-6 and IL-6 receptor in patients with renal osteodystrophy

AU - Langub, M. Chris

AU - Koszewski, Nicholas J.

AU - Turner, Heather V.

AU - Monier-Faugere, Marie Claude

AU - Geng, Zhaopo

AU - Malluche, Hartmut H.

N1 - Funding Information: Acknowledgments Funding for this project was supported in part by a grant from Dialysis Clinic Inc., Lexington, Kentucky (L-105,L-106). We are grateful to Dr. James P. Herman for allowing us to use the image analysis system and to Holli Gravatte, Kenloch F. Westberry III, and Richard M. Wheaton for valuable technical assistance. We thank Louise Tipton for her assistance in manuscript preparation.

PY - 1996

Y1 - 1996

N2 - The cytokine interleukin (IL-4) is a major cell regulation factor that may play an important role in the bone remodeling of patients renal failure. IL-6 exerts in action by binding to its receptor (IL-6R), which leads to transduction of a second messenger cascade within cells. In vitro as well as in vivo data point to IL-6 as an autocrine paracrine factor in bone osteoclasts. Recently, bone cells from patients with Paget's disease were found to express IL-6 and IL-6R mRNA transcripts. However, in patients with renal bone disease, there is currently no in vivo evidence that osteoclasts have the capability to express mRNA for IL-6 and IL-6R. To investigate the potential expression of IL-6 and IL-6R in bone and its relationship to bone cell activity, that crest bone biopsies were performed in patients on chronic maintenance dialysis. Messenger RNA expression of IL-4 and IL-6R was studied using in situ hybridization histochemistry, and parameters of bone turnover were determined by bone histomorphometry. In the samples studied, mRNA expression of IL-6 and IL-6R was found in osteoclasts and bone marrow cells. Furthermore, we report the novel finding of increased IL-6R mRNA expression in osteoclasts engaged in increased bone resorption. The results of the present study suggest that the cytokine IL-4 is intricately involved in osteoclastic bone resorption and that expression of its receptor. IL-65, in osteoclasts may parallel osteoclasts bone resorbing activity.

AB - The cytokine interleukin (IL-4) is a major cell regulation factor that may play an important role in the bone remodeling of patients renal failure. IL-6 exerts in action by binding to its receptor (IL-6R), which leads to transduction of a second messenger cascade within cells. In vitro as well as in vivo data point to IL-6 as an autocrine paracrine factor in bone osteoclasts. Recently, bone cells from patients with Paget's disease were found to express IL-6 and IL-6R mRNA transcripts. However, in patients with renal bone disease, there is currently no in vivo evidence that osteoclasts have the capability to express mRNA for IL-6 and IL-6R. To investigate the potential expression of IL-6 and IL-6R in bone and its relationship to bone cell activity, that crest bone biopsies were performed in patients on chronic maintenance dialysis. Messenger RNA expression of IL-4 and IL-6R was studied using in situ hybridization histochemistry, and parameters of bone turnover were determined by bone histomorphometry. In the samples studied, mRNA expression of IL-6 and IL-6R was found in osteoclasts and bone marrow cells. Furthermore, we report the novel finding of increased IL-6R mRNA expression in osteoclasts engaged in increased bone resorption. The results of the present study suggest that the cytokine IL-4 is intricately involved in osteoclastic bone resorption and that expression of its receptor. IL-65, in osteoclasts may parallel osteoclasts bone resorbing activity.

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Bone resorption and mRNA expression of IL-6 and IL-6 receptor in patients with renal osteodystrophy

Abstract

Bibliographical note

ASJC Scopus subject areas

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