Borrelia burgdorferi complement regulator-acquiring surface protein 2 (CSPZ) as a serological marker of human lyme disease

Peter Kraiczy, Annekatrin Seling, Catherine A. Brissette, Evelyn Rossmann, Klaus Peter Hunfeld, Tomasz Bykowski, Logan H. Burns, Matthew J. Troese, Anne E. Cooley, Jennifer C. Miller, Volker Brade, Reinhard Wallich, Sherwood Casjens, Brian Stevenson

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Serological diagnosis of Lyme disease may be complicated by antigenic differences between infecting organisms and those used as test references. Accordingly, it would be helpful to include antigens whose sequences are well conserved by a broad range of Lyme disease spirochetes. In the present study, line blot analyses were performed using recombinant complement regulator-acquiring surface protein 2 (BbCRASP-2) from Borrelia burgdorferi sensu stricto strain B31 and serum samples from human Lyme disease patients from throughout the United States and Germany. The results indicated that a large proportion of the patients had produced antibodies recognizing recombinant BbCRASP-2. In addition, Lyme disease spirochetes isolated from across North America and Europe were found to contain genes encoding proteins with high degrees of similarity to the B. burgdorferi type strain B31 BbCRASP-2, consistent with the high percentage of serologically positive patients. These data indicate that BbCRASP-2 may be valuable for use in a widely effective serological assay.

Original languageEnglish
Pages (from-to)484-491
Number of pages8
JournalClinical and Vaccine Immunology
Volume15
Issue number3
DOIs
StatePublished - Mar 2008

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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