TY - JOUR
T1 - Borrelia burgdorferi complement regulator-acquiring surface protein 2 (CSPZ) as a serological marker of human lyme disease
AU - Kraiczy, Peter
AU - Seling, Annekatrin
AU - Brissette, Catherine A.
AU - Rossmann, Evelyn
AU - Hunfeld, Klaus Peter
AU - Bykowski, Tomasz
AU - Burns, Logan H.
AU - Troese, Matthew J.
AU - Cooley, Anne E.
AU - Miller, Jennifer C.
AU - Brade, Volker
AU - Wallich, Reinhard
AU - Casjens, Sherwood
AU - Stevenson, Brian
PY - 2008/3
Y1 - 2008/3
N2 - Serological diagnosis of Lyme disease may be complicated by antigenic differences between infecting organisms and those used as test references. Accordingly, it would be helpful to include antigens whose sequences are well conserved by a broad range of Lyme disease spirochetes. In the present study, line blot analyses were performed using recombinant complement regulator-acquiring surface protein 2 (BbCRASP-2) from Borrelia burgdorferi sensu stricto strain B31 and serum samples from human Lyme disease patients from throughout the United States and Germany. The results indicated that a large proportion of the patients had produced antibodies recognizing recombinant BbCRASP-2. In addition, Lyme disease spirochetes isolated from across North America and Europe were found to contain genes encoding proteins with high degrees of similarity to the B. burgdorferi type strain B31 BbCRASP-2, consistent with the high percentage of serologically positive patients. These data indicate that BbCRASP-2 may be valuable for use in a widely effective serological assay.
AB - Serological diagnosis of Lyme disease may be complicated by antigenic differences between infecting organisms and those used as test references. Accordingly, it would be helpful to include antigens whose sequences are well conserved by a broad range of Lyme disease spirochetes. In the present study, line blot analyses were performed using recombinant complement regulator-acquiring surface protein 2 (BbCRASP-2) from Borrelia burgdorferi sensu stricto strain B31 and serum samples from human Lyme disease patients from throughout the United States and Germany. The results indicated that a large proportion of the patients had produced antibodies recognizing recombinant BbCRASP-2. In addition, Lyme disease spirochetes isolated from across North America and Europe were found to contain genes encoding proteins with high degrees of similarity to the B. burgdorferi type strain B31 BbCRASP-2, consistent with the high percentage of serologically positive patients. These data indicate that BbCRASP-2 may be valuable for use in a widely effective serological assay.
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U2 - 10.1128/CVI.00415-07
DO - 10.1128/CVI.00415-07
M3 - Article
C2 - 18160620
AN - SCOPUS:42049123508
SN - 1556-6811
VL - 15
SP - 484
EP - 491
JO - Clinical and Vaccine Immunology
JF - Clinical and Vaccine Immunology
IS - 3
ER -