Borrelia burgdorferi SpoVG DNA- and RNA-binding protein modulates the physiology of the Lyme disease spirochete

Christina R. Savage, Brandon L. Jutras, Aaron Bestor, Kit Tilly, Patricia A. Rosa, Yvonne Tourand, Philip E. Stewart, Catherine A. Brissette, Brian Stevensona

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The SpoVG protein of Borrelia burgdorferi, the Lyme disease spirochete, binds to specific sites of DNA and RNA. The bacterium regulates transcription of spoVG during the natural tick-mammal infectious cycle and in response to some changes in culture conditions. Bacterial levels of spoVG mRNA and SpoVG protein did not necessarily correlate, suggesting that posttranscriptional mechanisms also control protein levels. Consistent with this, SpoVG binds to its own mRNA, adjacent to the ribosome-binding site. SpoVG also binds to two DNA sites in the glpFKD operon and to two RNA sites in glpFKD mRNA; that operon encodes genes necessary for glycerol catabolism and is important for colonization in ticks. In addition, spirochetes engineered to dysregulate spoVG exhibited physiological alterations.

Original languageEnglish
Article numbere00033.18
JournalJournal of Bacteriology
Volume200
Issue number12
DOIs
StatePublished - Jun 1 2018

Bibliographical note

Publisher Copyright:
© 2018 American Society for Microbiology.

Keywords

  • Borrelia
  • DNA-binding proteins
  • RNA-binding proteins
  • Regulation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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