Brain creatine kinase with aging in F-344 rats: Analysis by saturation transfer magnetic resonance spectroscopy

C. D. Smith, W. Landrum, J. M. Carney, P. W. Landfield, M. J. Avison

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

We measured in vivo forward flux of the creatine kinase reaction in rat forebrain in young (Y: 6 month, n = 13), mid-aged (M: 12 month, n = 7) and aged (O: 27 month, n = 10) animals using 31P magnetic resonance saturation transfer. Forward flux was reduced in the aged rats (Y: 0.42 ± 0.08; M: 0.41 ± 0.10; O: 0.31 ± 0.03 s-1 ± SD; p = 0.008 O vs. Y). In vitro studies in a subset of the same rats showed a parallel decline in CK activity (Y: 2.16 ± 0.40; M: 2.17 ± 0.25; O: 1.56 ± 0.06 IU ±S.D.; p = 0.002 O vs. Y). The in vivo spectroscopic and in vitro biochemical measures were significantly correlated. Reduced creatine kinase activity could account for the observed decreased forward flux in aging brain. Intracellular pH, phosphocreatine/inorganic phosphate ratio, and phospocreatine/γ-adenosine triphosphate ratio did not differ between groups. Forward flux may represent a better measure of brain energy function than relative phosphocreatine or adenosine triphosphate levels observable in vivo.

Original languageEnglish
Pages (from-to)617-622
Number of pages6
JournalNeurobiology of Aging
Volume18
Issue number6
DOIs
StatePublished - Nov 1997

Bibliographical note

Funding Information:
This work was supported by NIA Grants POl-AG10836 and AG 07767.

Keywords

  • Aging
  • Creatine kinase
  • Magnetic resonance spectroscopy
  • Rat

ASJC Scopus subject areas

  • Neuroscience (all)
  • Aging
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

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