Brain Region Specific Single-Molecule Fluorescence Imaging

Xu Fu, Faruk H. Moonschi, Ashley M. Fox-Loe, Aaron A. Snell, Deann M. Hopkins, Alicia J. Avelar, Brandon J. Henderson, James R. Pauly, Christopher I. Richards

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

We developed an approach utilizing nanoscale vesicles extracted from brain regions combined with single molecule imaging to monitor how an animal's physiological condition regulates the dynamics of protein distributions in different brain regions. This method was used to determine the effect of nicotine on the distribution of receptor stoichiometry in different mouse brain regions. Nicotine-induced upregulation of α4β2 nicotinic acetylcholine receptors (nAChRs) is associated with changes in their expression, trafficking, and stoichiometry. The structural assembly of nAChRs has been quantified in cell culture based systems using single molecule techniques. However, these methods are not capable of quantifying biomolecule assembly that takes place in a live animal. Both nicotine-induced upregulation and changes in nAChR stoichiometry differ across brain regions. Our single molecule approach revealed that nicotine acts differentially across brain regions to alter assembly in response to exposure and withdrawal.

Original languageEnglish
Pages (from-to)10125-10131
Number of pages7
JournalAnalytical Chemistry
Volume91
Issue number15
DOIs
StatePublished - Aug 6 2019

Bibliographical note

Publisher Copyright:
© 2019 American Chemical Society.

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint

Dive into the research topics of 'Brain Region Specific Single-Molecule Fluorescence Imaging'. Together they form a unique fingerprint.

Cite this