Brain source imaging in preclinical rat models of focal epilepsy using high-resolution EEG recordings

Jihye Bae, Abhay Deshmukh, Yinchen Song, Jorge Riera

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Electroencephalogram (EEG) has been traditionally used to determine which brain regions are the most likely candidates for resection in patients with focal epilepsy. This methodology relies on the assumption that seizures originate from the same regions of the brain from which interictal epileptiform discharges (IEDs) emerge. Preclinical models are very useful to find correlates between IED locations and the actual regions underlying seizure initiation in focal epilepsy. Rats have been commonly used in preclinical studies of epilepsy; hence, there exist a large variety of models for focal epilepsy in this particular species. However, it is challenging to record multichannel EEG and to perform brain source imaging in such a small animal. To overcome this issue, we combine a patented-technology to obtain 32-channel EEG recordings from rodents and an MRI probabilistic atlas for brain anatomical structures in Wistar rats to perform brain source imaging. In this video, we introduce the procedures to acquire multichannel EEG from Wistar rats with focal cortical dysplasia, and describe the steps both to define the volume conductor model from the MRI atlas and to uniquely determine the IEDs. Finally, we validate the whole methodology by obtaining brain source images of IEDs and compare them with those obtained at different time frames during the seizure onset.

Original languageEnglish
Article numbere52700
JournalJournal of Visualized Experiments
Volume2015
Issue number100
DOIs
StatePublished - Jun 6 2015

Bibliographical note

Publisher Copyright:
© 2015 Journal of Visualized Experiments.

Keywords

  • Brain source imaging
  • Electroencephalography (EEG)
  • Epilepsy
  • Issue 100
  • Medicine
  • Neuroscience
  • Preclinical model
  • Rodents

ASJC Scopus subject areas

  • General Neuroscience
  • General Chemical Engineering
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology

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