Burkholderia multivorans requires species-specific GltJK for entry of a contact-dependent growth inhibition system protein

Tanya Myers-Morales, Martha M.S. Sim, Tanner J. DuCote, Erin C. Garcia

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Interbacterial antagonism and communication are driving forces behind microbial community development. In many Gram-negative bacteria, contact-dependent growth inhibition (CDI) systems contribute to these microbial interactions. CDI systems deliver the toxic C-terminus of a large surface exposed protein to the cytoplasm of neighboring bacteria upon cell−contact. Termed the BcpA-CT, import of this toxic effector domain is mediated by specific, yet largely unknown receptors on the recipient cell outer and inner membranes. In this study, we demonstrated that cytoplasmic membrane proteins GltJK, components of a predicted ABC-type transporter, are required for entry of CDI system protein BcpA-2 into Burkholderia multivorans recipient cells. Consistent with current CDI models, gltJK were also required for recipient cell susceptibility to a distinct BcpA-CT that shared sequences within the predicted “translocation domain” of BcpA-2. Strikingly, this translocation domain showed low sequence identity to the analogous region of an Escherichia coli GltJK-utilizing CDI system protein. Our results demonstrated that recipient bacteria expressing E. coli gltJK were resistant to BcpA-2-mediated interbacterial antagonism, suggesting that BcpA-2 specifically recognizes Burkholderia GltJK. Using a series of chimeric proteins, the specificity determinant was mapped to Burkholderia-specific sequences at the GltK C-terminus, providing insight into BcpA transport across the recipient cell cytoplasmic membrane.

Original languageEnglish
Pages (from-to)957-973
Number of pages17
JournalMolecular Microbiology
Volume116
Issue number3
DOIs
StatePublished - Sep 2021

Bibliographical note

Funding Information:
This work was supported by grants from the National Institutes of Health (K22AI118949 and R01AI150767 to E.C.G) and start‐up funding from the University of Kentucky. We thank Zaria Elery (University of Kentucky) for technical assistance.

Publisher Copyright:
© 2021 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

Keywords

  • CDI
  • bacterial antagonism
  • bacterial competition

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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