Burkholderia thailandensis is aGram-negative bacterium endemic to Southeast Asian and northern Australian soils. It is non-pathogenic; therefore, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei. B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini-Tn7 insertion, and allelic exchange in B. thailandensis.
|Journal||Current Protocols in Microbiology|
|State||Published - May 1 2017|
Bibliographical notePublisher Copyright:
© 2017 John Wiley & Sons, Inc.
- Allelic exchange
- Transformation burkholderia thailandensis
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