Burkholderia thailandensis: Genetic manipulation

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6 Scopus citations

Abstract

Burkholderia thailandensis is aGram-negative bacterium endemic to Southeast Asian and northern Australian soils. It is non-pathogenic; therefore, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei. B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini-Tn7 insertion, and allelic exchange in B. thailandensis.

Original languageEnglish
Pages (from-to)4C.2.1-4C.2.15
JournalCurrent Protocols in Microbiology
Volume2017
DOIs
StatePublished - May 1 2017

Bibliographical note

Publisher Copyright:
© 2017 John Wiley & Sons, Inc.

Keywords

  • Allelic exchange
  • Conjugation
  • Mini-tn7
  • Mutation
  • Transformation burkholderia thailandensis

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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