Abstract
Burkholderia thailandensis is aGram-negative bacterium endemic to Southeast Asian and northern Australian soils. It is non-pathogenic; therefore, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei. B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini-Tn7 insertion, and allelic exchange in B. thailandensis.
| Original language | English |
|---|---|
| Pages (from-to) | 4C.2.1-4C.2.15 |
| Journal | Current Protocols in Microbiology |
| Volume | 2017 |
| DOIs | |
| State | Published - May 1 2017 |
Bibliographical note
Publisher Copyright:© 2017 John Wiley & Sons, Inc.
Funding
| Funders | Funder number |
|---|---|
| National Institute of Allergy and Infectious Diseases | K22AI118949 |
Keywords
- Allelic exchange
- Conjugation
- Mini-tn7
- Mutation
- Transformation burkholderia thailandensis
ASJC Scopus subject areas
- Parasitology
- Microbiology
- Virology